PNNL

Abstract

In general, classification-based methods based on Confocal Raman microscopy are focused on targeted studies under which the spectral libraries are collected under controlled instrument parameters, which facilitate analyses via standard multivariate data analysis methods and cross-validation. We develop and compare approaches to combine spectra collected at different times and varying levels of spectral resolution into a single spectral library. We demonstrate these approaches on a relevant test case; the identification of microbial species from a natural environment.