Oxygenic photosynthesis is initiated by photosystem II (PSII) in the thylakoid membranes of plants, algae and cyanobacteria. PSII is a multi-subunit pigment-protein complex responsible for splitting water into oxygen gas, hydrogen ions, and electrons transferred to electron acceptors during photosynthesis.1 Two homologous membrane-spanning proteins D1 (PsbA) and D2 (PsbD) form the PSII complex core.1 Peripherally, two chlorophyll (Chl)-binding inner antenna proteins CP47 (PsbB) and CP43 (PsbC) are bound to the D1-D2 PSII complex core.1 These four large proteins are surrounded by a large number of smaller membrane proteins.2 Most of these small proteins have been observed in the crystal structures of the PSII complex from cyanobacteria.3,4 However, one small protein, Psb28, previously detected as a nonstoichiometric component of PSII,5 was not observed in the crystal structures indicating that Psb28 might not be a true PSII subunit. Recent studies revealed that Psb28 was preferentially bound to PSII core complex lacking CP43 (RC47) and involved in the biogenesis of CP47.6 Understanding the association of Psb28 to PSII core complex should provide additional insight into its role in PSII function, however, the structure of Psb28 remains unknown. In this note, we report the solution NMR structure of Psb28 protein encoded by gene sll1398 [gi|952386] of Synechocystis sp. strain PCC 6803(SWISS-PROT ID: PSB28_SYNY3, NESG target ID: SgR171).7 This protein, also named Psb13 or ycf79, belongs to the Psb28 protein family (Pfam ID: PF03912), which is currently made up of ~48 protein sequences. Both PSI-BLAST and Dali8 searches indicated this is the first atomic resolution structure available for the Psb28 family. ConSurf9 was used to identify conserved residues potentially involved in binding to the PSII core complex.10
Revised: April 7, 2011 |
Published: December 15, 2010
Citation
Yang Y., T.A. Ramelot, J.R. Cort, D. Wang, C. Ciccosanti, K. Hamilton, and R. Nair, et al. 2010.Solution NMR Structure of Photosystem II Reaction Center Protein Psb28 from Synechocystin sp. Strain PCC 6803.Proteins. Structure, Function, and Bioinformatics 79, no. 1:340-344. PNWD-SA-8966. doi:10.1002/prot.22876