Differential ion mobility spectrometry (FAIMS) is emerging as a broadly useful tool for separation of isomeric modified peptides with post-translational modifications (PTMs) attached to alternative residues. Such separations were anticipated to become more challenging for smaller PTMs and longer peptides. Here we show that FAIMS can fully resolve localization variants involving a PTM as minuscule as methylation, even for larger peptides in the middle-down range.
Revised: August 9, 2012 |
Published: July 18, 2012
Citation
Shvartsburg A.A., Y. Zheng, R.D. Smith, and N. Kelleher. 2012.Separation of Variant Methylated Histone Tails by Differential Ion Mobility.Analytical Chemistry 84, no. 15:6317-6320.PNNL-SA-88466.doi:10.1021/ac301541r