The behavior of proteins on solids has been studied over several decades. Recent developments have expanded the variety of methods that can be used to examine protein-surface interactions. This paper summarizes the adsorptive behavior of proteins on various surfaces including cotton and synthetic polymers. In general, it is postulated that surfaces act like catalysts for protein unfolding. We give specific examples of the unfolding of cytochrome c and show a lowering of the unfolding temperature from solution to an anionic surface. Additionally, we show how chromatography can be used to screen adsorbents, such as cotton for possible commercial applications. The ability of each surface chemistry to act as a catalyst for unfolding is discussed.
Revised: May 4, 2004 |
Published: March 1, 2001
Citation
Goheen S.C., B.M. Gibbins, J.L. Hilsenbeck, and J.V. Edwards. 2001.Retention, Unfolding, and Deformation of Soluble Proteins on Solids. In Bioactive Fibers and Polymers. ACS Symposium Series, edited by J. Vincent Edwards and Tyrone L. Vigo, 792, 20-34. Washington, District Of Columbia:American Chemical Society.PNNL-SA-34154.