Sandwich ELISA microarrays have the potential to simultaneously quantify the levels of multiple diagnostic targets in a biological sample. However, as seen with traditional ELISA diagnostics, heterophilic antibodies (HA) in patient sera have the potential to cause interference in these assays. We demonstrate here that reducing the diagnostic capture antibody to its minimal functional unit, the variable heavy and light domains artificially connected with a short polypeptide linker (scFv), is an effective strategy for reducing the HA assay interference.
Revised: November 4, 2011 |
Published: December 15, 2011
Citation
Baird C.L., R. Tan, C.J. Fischer, K.D. Victry, R.C. Zangar, and K.D. Rodland. 2011.Reducing heterophilic antibody interference in immunoassays using single chain antibodies.Analytical Biochemistry 419, no. 2:333-335.PNNL-SA-77526.doi:10.1016/j.ab.2011.08.025