August 6, 2015
Journal Article

Reagent-free and portable detection of Bacillus anthracis spores using a microfluidic incubator and smartphone microscope

Abstract

Rapid, cost-effective bacterial detection systems are needed to respond to potential biothreat events. Here we report the use of smartphone-based microscopy in combination with a simple microfluidic incubation device to detect 5000 Bacillus anthracis spores in 3 hours. This field-deployable approach is compatible with real-time PCR for secondary confirmation.

Revised: November 12, 2015 | Published: August 6, 2015

Citation

Hutchison J.R., R.L. Erikson, A.M. Sheen, R.M. Ozanich, and R.T. Kelly. 2015. Reagent-free and portable detection of Bacillus anthracis spores using a microfluidic incubator and smartphone microscope. Analyst 140, no. 18:6269-6276. PNNL-SA-109030. doi:10.1039/C5AN01304F