Quantitative live cell mass spectrometry analysis at a subcellular
level requires the precisely controlled extraction of subpicoliter volumes of material
from the cell, sensitive analysis of the extracted analytes, and their accurate
quantification without prior separation. In this study, we demonstrate that localized
electroosmotic extraction provides a direct path to addressing this challenge.
Specifically, we demonstrate quantitative mass spectrometry analysis of
biomolecules in picoliter volumes extracted from live cells. Electroosmotic
extraction was performed using two electrodes and a finely pulled nanopipette
with tip diameter of
Revised: November 5, 2019 |
Published: July 3, 2018
Citation
Yin R., V. Prabhakaran, and J. Laskin. 2018.Quantitative Extraction and Mass Spectrometry Analysis at a Single-Cell Level.Analytical Chemistry 90, no. 13:7937-7945.PNNL-SA-137460.doi:10.1021/acs.analchem.8b00551