The continued fast pace of fungal genome sequence generation has enabled proteomic analysis of a wide breadth of organisms that span the breadth of the Kingdom Fungi. There is some phylogenetic bias to the current catalog of fungi with reasonable DNA sequence databases (genomic or EST) that could be analyzed at a global proteomic level. However, the rapid development of next generation sequencing platforms has lowered the cost of genome sequencing such that in the near future, having a genome sequence will no longer be a time or cost bottleneck for downstream proteomic (and transcriptomic) analyses. High throughput, non-gel based proteomics offers a snapshot of proteins present in a given sample at a single point in time. There are a number of different variations on the general method and technologies for identifying peptides in a given sample. We present a method that can serve as a “baseline” for proteomic studies of fungi.
Revised: August 24, 2011 |
Published: April 20, 2011
Citation
Baker S.E., and E.A. Panisko. 2011.Proteome Studies of Filamentous Fungi. In Fungal Genomics: Methods and Protocols. Methods in Molecular Biology, edited by JR Xu and BHH Bluhm. 133-139. New York, New York:Springer.PNNL-SA-78038.