The global proteomic response of Arthrobacter sp. strain FB24 to different levels of chromate stress was evaluated with both two-dimensional gel electrophoresis (2-DGE) and liquid chromatography coupled to tandem mass spectrometry (LC/LC-MS/MS) [Henne et al. 2009b]. Proteome coverage of 22% and 71% was obtained with 2-DGE and LC/LC-MS/MS, respectively. The strong response of strain FB24 to chromate suggests a condition of sulfur limitation, which could be driven by competition for the sulfate transporter by the structurally similar chromate ion. Additionally, the involvement of genes hypothesized to be directly involved in chromate resistance in strain FB24 was supported at the protein level.
Revised: January 10, 2012 |
Published: May 3, 2011
Citation
Henne K.L., J.E. Turse, C.H. Nakatsu, and A. Konopka. 2011.Protein Expression Profile of an Environmentally Important Bacterial Strain: the Chromate Response of Arthrobacter Species Strain FB24. In Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches, edited by FJ de Bruijn. 663–674. Hoboken, New Jersey:John Wiley & Sons, Inc.PNNL-SA-75447.