We are developing nuclear magnetic resonance (NMR) microscopy, spectroscopy and combined NMR/optical techniques to the study of biofilms. Objectives include: time and depth-resolved metabolite concentrations with isotropic spatial resolution on the order of 10 microns, metabolic pathways and flux rates, mass transport and ultimately their correlation with gene expression by optical microscopy in biofilms. These methods are being developed with Shewanella oneidensis MR-1 as a model system, but are equally applicable to other biofilm systems of interest. Thus, spatially resolved NMR of biofilms is expected to contribute significantly to the understanding of adherent cell metabolism.
Revised: November 22, 2005 |
Published: November 1, 2005
Citation
Majors P.D., J.S. Mclean, J.K. Fredrickson, and R.A. Wind. 2005.NMR methods for in-situ biofilm metabolism studies: spatial and temporal resolved measurements.Water Science and Technology 52, no. 7:7-12.PNNL-SA-43300.