August 1, 2006
Journal Article

Multispectral/hyperspectral image enhancement for biological cell analysis

Abstract

The paper shows new techniques for analyzing cell images taken with a microscope using multiple filters to form a datacube of spectral image planes. Because of the many neighboring spectral samples, much of the datacube appears as redundant, similar tissue. The analysis is based on the nonGaussian statistics of the image data, allowing for remapping of the data into image components that are dissimilar, and hence isolate subtle, spatial object regions of interest in the tissues. This individual component image set can be recombined into a single RGB color image useful in real-time location of regions of interest. The algorithms are susceptible to parallelization using Field Programmable Gate Array hardware processing.

Revised: December 22, 2011 | Published: August 1, 2006

Citation

Nuffer L.L., P.A. Medvick, H.P. Foote, and J.C. Solinsky. 2006. Multispectral/hyperspectral image enhancement for biological cell analysis. Cytometry. Part A 69, no. 8:897-903. PNNL-SA-46611. doi:10.1002/cyto.a.20294