July 21, 2014
Journal Article

A Method to determine lysine acetylation stoichiometries

Abstract

A major bottleneck to fully understanding the functional aspects of lysine acetylation is the lack of stoichiometry information. Here we describe a mass spectrometry method using a combination of isotope labeling and detection of a diagnostic fragment ion to determine the stoichiometry of lysine acetylation on proteins globally. Using this technique, we determined the modification occupancy on hundreds of acetylated peptides from cell lysates and cross-validated the measurements via immunoblotting.

Revised: February 19, 2015 | Published: July 21, 2014

Citation

Nakayasu E.S., S. Wu, M.A. Sydor, A.K. Shukla, K.K. Weitz, R.J. Moore, and K.K. Hixson, et al. 2014. A Method to determine lysine acetylation stoichiometries. International Journal of Proteomics 2014. PNNL-SA-96571. doi:10.1155/2014/730725