Biomarkers potentially play a crucial role in early disease diagnosis, prognosis and targeted therapy. In the past decade, mass spectrometry based proteomics has become increasingly important in biomarker development due to large advances in technology and associated methods. This chapter mainly focuses on the application of broad (e.g. shotgun) proteomics in biomarker discovery and the utility of targeted proteomics in biomarker verification and validation. A range of mass spectrometry methodologies are discussed emphasizing their efficacy in the different stages in biomarker development, with a particular emphasis on blood biomarker development.
Revised: July 28, 2015 |
Published: June 19, 2015
Citation
Wu C., T. Liu, E.S. Baker, K.D. Rodland, and R.D. Smith. 2015.Mass spectrometry for biomarker development. In General Methods in Biomarker Research and their Applications. Biomarkers in Disease: Methods, Discoveries and Applications, edited by V Preedy and V Patel. 17-48. Heidelberg:Springer.PNNL-SA-97608.