Differential ion mobility spectrometry (FAIMS) can baseline-resolve multiple variants of post-translationally modified peptides extending to the 3 - 4 kDa range, which differ in the localization of a PTM as small as acetylation. Essentially orthogonal separations for different charge states expand the total achievable peak capacity in proportion to the number of observed states that increases for longer polypeptides. This might enable resolving localization variants for even larger peptides and intact proteins.
Revised: May 30, 2012 |
Published: May 15, 2012
Citation
Shvartsburg A.A., Y. Zheng, R.D. Smith, and N. Kelleher. 2012.Ion Mobility Separation of Variant Histone Tails Extending to the “Middle-down” Range.Analytical Chemistry 84, no. 10:4271-4276.PNNL-SA-86196.doi:10.1021/ac300612y