Nuisance factors in a protein-array study add obfuscating variation to spot intensity measurements, diminishing the accuracy and precision of protein concentration predictions. The effects of nuisance factors may be reduced by design of experiments, and by estimating and then subtracting nuisance effects. Estimated nuisance effects also inform about the quality of the study and suggest refinements for future studies.
Revised: September 1, 2010 |
Published: January 28, 2010
Citation
Daly D.S., K.K. Anderson, S.L. Seurynck-Servoss, R.M. Gonzalez, A.M. White, and R.C. Zangar. 2010.An internal calibration method for protein-array studies.Statistical Applications in Genetics and Molecular Biology 9, no. 1:Article Number: 14.PNNL-SA-67909.