We demonstrate here the use of natural abundance isotopic "labels" to aid in the identification of peptides with a de novo algorithm. Using data from ion trap MS/MS analysis of 102 tryptic peptides, we analyze multiple series of peaks within MS/MS spectra that "spell" peptide sequences. Isotopic peaks from naturally abundant isotopes are particularly prominent on y and b series ions and lead to an increased confidence in the identification of the precursor peptides. Sequence analysis of the MS/MS data is accomplished by finding sequences and subsequences in a hierarchical manner within the spectra.
Revised: September 3, 2003 |
Published: June 27, 2003
Citation
Cannon W.R., and K.D. Jarman. 2003.Improved peptide sequencing using isotope information inherent in tandem mass spectra.Rapid Communications in Mass Spectrometry 17, no. 15:1793-1801.PNNL-SA-36888.