PNNL

Abstract

By synchronizing electrochemical potential scanning with a single-molecule localization super-resolution fluorescence microscope, kinetic fluorescence changes of hundreds of single molecular redox events were tracked simultaneously in high throughput and subsequent cross-correlation function analysis mapped single molecules’ redox potentials (times) out on the imaging area from site to site in an unprecedented detail by extracting electrochemically-induced fluorescence change from apparently random fluorescence on/off blinking. This work paves the way towards mapping redox states at single-molecule levels in high throughput in chemical and biological systems.