Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection and validation of disease biomarkers. ELISA microarrays are capable of simultaneous detection of many proteins using a small sample volume. Although there are many potential pitfalls to the use of ELISA microarrays, these can be avoided by careful planning of experiments. In this chapter we describe a high-throughput protocol for processing ELISA microarrays that will result in reliable and reproducible data.
Revised: June 11, 2009 |
Published: May 11, 2009
Citation
Servoss S., R.M. Gonzalez, S.M. Varnum, and R.C. Zangar. 2009.High-Throughput Analysis of Serum Antigens Using Sandwich ELISAs on Microarrays. In Tumor Biomarker Discovery, Methods in Molecular Biology. 143-150. Totowa, New Jersey:Humana Press.PNNL-SA-55713.