There is an unmet technical challenge for mass spectrometry-based proteomics analysis of single mammalian cells. Quantitative proteomic analysis of single cells has been exclusively achieved by antibody-based immunoassays but is limited by the availability of high-quality antibodies. Herein we report a facile targeted mass spectrometry-based proteomics method for analysis of 1-100 human cells with the sensitivity to reliably detect proteins at =3,000 and =200,000 copies per cell in 100 cells and within a single cell, respectively. The method can be easily implemented in any mass spectrometry and proteomics laboratory without the need for specialized instrumentation or reagents.
Revised: August 14, 2019 |
Published: August 6, 2018
Citation
Shi T., M.J. Gaffrey, T.L. Fillmore, C.D. Nicora, L. Yi, P. Zhang, and A.K. Shukla, et al. 2018.Facile carrier-assisted targeted mass spectrometric approach for proteomic analysis of low numbers of mammalian cells.Communications Biology 1, no. 1:Article No. 103.PNNL-SA-130600.doi:10.1038/s42003-018-0107-6