Heterologous expression of the A. niger NR-PKS gene, e_gw1_19.204 and the adjacent stand-alone R domain gene, est_GWPlus_C_190476 in A. nidulans demonstrated that they belong to a single gene named dtbA. The DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde 1 and 2-ethyl-4,6-dihydroxy-3,5-dimethylbenzaldehyde 2. Generation of DtbA?R-TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.
Revised: March 4, 2013 |
Published: January 31, 2013
Citation
Yeh H., S. Chang, Y.M. Chiang, K.S. Bruno, B.R. Oakley, T. Wu, and C.C. Wang. 2013.Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping.Organic Letters 15, no. 4:756-759.PNNL-SA-92219.doi:10.1021/ol303328t