PNNL

Abstract

Objective: Physiological and biochemical processes across tissues of the body are regulated in response to the unique demands of intense physical activity. A better understanding of such processes can ultimately help improve human performance and prevent illnesses in the work environment. Methods: To study regulatory processes in intense physical activity simulating real life conditions, we performed a multi-omics analysis of three biofluids (blood plasma, urine and saliva) collected from firefighters before and after a 45-minute, intense exercise regimen. Results: The blood plasma analysis revealed signatures of tissue damage and acute repair response accompanied by enhanced carbon metabolism to meet energy demands. The urine analysis showed a strong, concomitant regulation of the renin-angiotensin system supporting increased excretion of catabolites, reabsorption of nutrients and maintenance of fluid balance. In saliva, we observed a decrease in inflammatory molecules and an increase in antimicrobial peptides. A systematic literature review suggested an altered susceptibility to respiratory infection. Conclusions: This study shows simultaneous regulatory signatures in biofluids indicative of homeostatic maintenance during intense physical activity with possible effects on increased infection susceptibility.