A disposable sensor for the determination of cotinine in human serum was developed based on immunochromatographic test strip and quantum dot label. In this assay, cotinine modified on quantum dot competes with cotinine in sample to bind to anti-cotinine antibody in the test strip and the quantum dots serve as signal vehicles for electrochemical readout. Some parameters governing the performance of the sensor were optimized. The sensor shows a wide linear range from 1 ng mL-1 to 100 ng mL-1 cotinine with a detection limit of 1.0 ng mL-1. The relative standard deviation (R.S.D.) of the sensor was less than 2% for cotinine. The sensor was validated with spiked human serum samples and it was found that this method was reliable in measuring cotinine in human serum with average recovery of 100.99%. The results demonstrate that this sensor is a rapid, clinically accurate, and less expensive and has the potential for point of care (POC) detection of cotinine and fast screening of tobacco smoke exposure.
Revised: March 15, 2012 |
Published: February 3, 2012
Citation
Nian H., J. Wang, H.J. Wu, J. Lo, K. Chiu, J.G. Pounds, and Y. Lin. 2012.Electrochemical Immunoassay of Cotinine in Serum Based on Nanoparticle Probe and Immunochromatographic Strip.Analytica Chimica Acta 713.PNNL-SA-82060.doi:10.1016/j.aca.2011.11.028