Differential ion mobility spectrometry (IMS) or field asymmetric waveform IMS (FAIMS) sorts gas-phase ions by mobility differences with respect to the electric field intensity. A major emerging FAIMS application is the fractionation of proteolytic digests. Using a planar FAIMS unit with helium/nitrogen mixtures, we have increased FAIMS resolving powers for peptide analyses from the prior maximum of ~20 - 30 to ~50 - 70. The resolution improved nearly 3-fold, allowing, in particular, separation of previously unresolved conformers.
Revised: July 22, 2010 |
Published: January 1, 2010
Citation
Shvartsburg A.A., K. Tang, and R.D. Smith. 2010.Differential Ion Mobility Separations of Peptides with Resolving Power Exceeding 50.Analytical Chemistry 82, no. 1:32-35. PNWD-SA-8758. doi:10.1021/ac902133n