PNNL

Abstract

Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells, and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 targets synthesis. In this study we designed anti-mir-10b molecules and combined with poly L-lysine (PLL) nanoparticles to test the delivery effectiveness. RNA molecule sequence exactly matching the mature mir-10b minor antisense showed strong inhibition when mixed with PLL in our wound healing assay. In addition the nanoparticles deliver the anti-microRNA molecules into cytoplasm of breast cancer cells in a concentration dependent manor which are sustainable for long term and slow-release effectiveness.