January 12, 2022
Journal Article

Contrasting community assembly forces drive microbial structural and functional responses to precipitation in an incipient soil system

Abstract

Microbial communities in incipient soil systems serve as the only biotic force shaping landscape evolution. However, the underlying ecological forces shaping microbial community structure and function are inadequately understood. We used amplicon sequencing to determine microbial taxonomic assembly and metagenome sequencing to evaluate microbial functional assembly in incipient basaltic soil subjected to precipitation. Community composition was stratified with soil depth in the pre-precipitation samples, with surficial communities maintaining their distinct structure and diversity after precipitation, while the deeper soil samples appeared to become more uniform. The structural community assembly remained deterministic in pre- and post precipitation periods, with homogenous selection being dominant. Metagenome analysis revealed that carbon and nitrogen functional potential was assembled stochastically. Sub populations putatively involved in the nitrogen cycle and carbon fixation experienced counteracting assembly pressures at the deepest depths, suggesting the communities may functionally assemble to respond to short-term environmental fluctuations and impact the landscape-scale response to perturbations. We propose that contrasting assembly forces impact microbial structure and function in an incipient landscape; in situ landscape characteristics (here homogenous parent material) drive community structure assembly while short-term environmental fluctuations (here precipitation) shape environmental variations that are random in the soil depth profile and drive stochastic sub-population functional dynamics.

Published: January 12, 2022

Citation

Sengupta A., T. Volkmann, R.E. Danczak, J.C. Stegen, K. Dontsova, N. Abramson, and A. Bugaj, et al. 2021. "Contrasting community assembly forces drive microbial structural and functional responses to precipitation in an incipient soil system." Frontiers in Microbiology 12. PNNL-SA-166658. doi:10.3389/fmicb.2021.754698