In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated “Ente-robacter lignolyticus” SCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are net methane producers. Because of its ability to grow on lignin anae-robically, we sequenced the genome. The genome of “E. lignolyticus” SCF1 is 4.81 Mbp with no detected plasmids, and includes a relatively small arsenal of lignocellulolytic carbohy-drate active enzymes. Lignin degradation was observed in culture, and the genome revealed two putative laccases, a putative peroxidase, and a complete 4-hydroxyphenylacetate degra-dation pathway encoded in a single gene cluster.
Revised: May 31, 2012 |
Published: September 23, 2011
Citation
DeAngelis K.M., P. D'Haeseleer, D. Chivian, J.L. Fortney, J.I. Khudyakov, B.A. Simmons, and H. Woo, et al. 2011.Complete genome sequence of “Enterobacter lignolyticus” SCF1.Standards in Genomic Sciences 5, no. 1:69-85. doi:10.4056/sigs.2104875