We report on the development and characterization of an automated metal-free nanoscale multiple-capillary system for reversed-phase liquid chromatography-mass spectrometry analysis of phosphopeptides. The system incorporates a capillary column (50 um i.d. × 30 cm, packed with 5 um C18 particles) coupled on-line to a solid phase extraction column (150 um i.d. × 4 cm, packed with 5 um C18 particles). Electrospray ionization tips are constructed on the packed capillary column to couple the reversed-phase liquid chromatographic separation to a linear ion trap tandem mass spectrometer.
Revised: April 24, 2009 |
Published: March 15, 2009
Citation
Zhao R., S. Ding, Y. Shen, D.G. Camp, E.A. Livesay, H.R. Udseth, and R.D. Smith. 2009.Automated metal-free multiple-column nanoLC for improved phosphopeptide analysis sensitivity and throughput.Journal of Chromatography B 877, no. 8-9:663-670.PNNL-SA-62634.