PNNL

Abstract

In this communication, we report the illustration of cellular delivering and in situ molecular probing in living cells by using graphene oxide nanosheets (GO-nS) as DNA cargo and sensing platform. Due to the particular interaction between GO and DNA molecules, aptamer/GO-nS nanocomplex was designed and employed to demonstrate the dramatic DNA delivering, enzymatic cleavage protecting and biosensing capabilities of GO-nS in living cells. The results show that GO-nS could successfully transfer DNA aptamer into living cells, efficiently protect oligonucleotides from enzymatic cleavage during the delivery as well as selectively monitoring intra-cellular target in situ. The as-shown advantages of graphene oxide will enable it to be an robust candidate for many biological fields, such as DNA and protein analyzing, gene and drug delivering, intracellular tracking, and in vivo monitoring, etc..