Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillusterreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP related melanin synthetase was also identified in this species.
Revised: March 27, 2015 |
Published: July 8, 2013
Citation
Guo C., B.P. Knox, J.F. Sanchez, Y. Chiang, K.S. Bruno, and C.C. Wang. 2013.Application of an Efficient Gene Targeting System Linking Secondary Metabolites to their Biosynthetic Genes in Aspergillus terreus.Organic Letters 15, no. 14:3562-3565.PNNL-SA-95505.doi:10.1021/ol401384v