PNNL

Vince Gerbasi is a microbiologist in the Exposure and Pathogen Biology group at PNNL. His research uses high-resolution mass spectrometry to study infectious diseases and host responses.

• Postdoctoral Fellowship, Molecular Biosciences, Northwestern University, 2010
• PhD in Microbiology and Immunology, Vanderbilt University Medical School, 2007
• BS in Microbiology, Washington State University, 2002

2022

• Melani RD, Gerbasi VR, Anderson LC, Sikora JW, Toby TK, Hutton JE, Butcher DS, Negrão F, Seckler HS, Srzentić K, Fornelli L, Camarillo JM, LeDuc RD, Cesnik AJ, Lundberg E, Greer JB, Fellers RT, Robey MT, DeHart CJ, Forte E, Hendrickson CL, Abbatiello SE, Thomas PM, Kokaji AI, Levitsky J, Kelleher NL. The Blood Proteoform Atlas: A Reference Map of Proteoforms in Human Hematopoietic Cells. 2022 Science 375, 6579, 411-418.
• Lisa Bramer, Robert Hontz, Amie Eisfeld, Amy Sims, Young-Mo Kim, Kelly Stratton, Carrie Nicora, Marina Gritsenko, Athena Schepmoes, Osamu Akasaka, Michiko Koga, Takeya Tsutsumi, Morio Nakamura, Ichiro Nakachi, Rie Baba, Hiroki Tateno, Shoji Suzuki, Hideaki Nakajima, Hideaki Kato, Kazunari Ishida, Makoto Ishii, Yoshifumi Uwamino, Keiko Mitamura, Venessa Paurus, Ernesto Nakayasu, Isaac Attah, Andrew G Letizia, Katrina Waters, Tom Metz, Karen Corson, Yoshihiro Kawaoka, Vincent R Gerbasi. Multi-omics Characterization of Neutrophil Extracellular Trap Formation in Severe and Mild COVID-19 Infections. 2022 MedRxiv doi.org/10.1101/2022.04.26.22274196

• Jesse W. Wilson, Aivett Bilbao, Juan Wang, Yen-Chen Liao, Dusan Velickovic, Roza Wojcik, Marta Passamonti, Rui Zhao, Andrea Gargano, Vincent R. Gerbasi, Ljiljana Pasa-Tolic, Scott Baker, Mowei Zhou. Online. Hydrophilic Interaction Chromatography (HILIC) Enhanced Top-Down Mass Spectrometry Characterization of the SARS-CoV-2 Spike Receptor-Binding Domain. 2022 Analytical Chemistry 94, 15, 5909–5917.

2021

• Gerbasi VR, Melani RD, Abbatiello SE, Belford MW, Huguet R, McGee JP, Dayhoff D, Thomas PM,  Kelleher NL.  Deeper Protein Identification Using Field Asymmetric Ion Mobility Spectrometry in Top-Down Proteomics.  2021 Anal. Chem. 93, 16, 6323–6328

2020

• Link AJ, Niu X, Weaver CM, Jennings JL, Duncan DT, McAfee KJ, Sammons M, Gerbasi VR, Farley AR, Fleischer TC, Browne CM, Samir P, Galassie A, Boone B. Targeted Identification of Protein Interactions in Protein Translation. 2020 Proteomics PMID: 32027465
• Winer B, Edgel KA, Zou X, Sellau J, Hadiwidjojo S, Garver LS, McDonough CE, Kelleher NL, Thomas PM, Villasante E, Ploss A, Gerbasi VR.Identification of Plasmodium falciparum Proteoforms from Liver Stage Models. 2020 Malaria J.19:10.

2019

• Cawlfield A, Genito CJ, Beck Z, Bergmann-Leitner ES, Bitzer AA, Soto K, Zou X, Hadiwidjojo SH, Gerbasi RV, Mullins AB, Noe A, Waters NC, Alving CR, Matyas GR, Dutta S. (2019) Safety, Toxicity, and immunogenicity of a malaria vaccine based on the circumsporozoite protein (FMP013) with the adjuvant army liposome formulation containing QS21(ALFQ). Vaccine, Jun 27;37(29):3793-3803.
• Des Soye BJ, Gerbasi VR, Thomas PM, Kelleher NL, Jewett MC. A Highly Productive, One-Pot Cell-free Protein Synthesis Platform Based on Genomically Recoded Escherichia Coli.  2019 Cell Chem Bio. Nov 6; S2451-9456 (19)30553-8.
• Melani RD, Srzentić K, Gerbasi VR, McGee JP, Huguet R, Fornelli L, and Kelleher NL. Direct Measurement of Light and Heavy Antibody Chains Using FAIMS and Middle-Down Mass Spectrometry. (2019) mAbs. Nov-Dec; 11(8): 1351-1357.

2018

• Gerbasi VR, Browne CM, Samir P, Shen B, Sun M, Hazelbaker DZ, Galassie AC, Frank J, Link AJ. (2018) Critical role of Saccharomyces cerevisiae Asc1p in translational initiation at elevated temperatures. Proteomics, Oct 4:e1800208. doi: 10.1002/pmic.201800208.

2017

• Cowell, AN., Loy, DE., Saundararaman, SA., Valdivia, H., Fisch, K., Lescano AG, Baldeviano, GC, Durand S., Gerbasi V., Sutherland CJ, Nolder D., Vinetz JM, Hahn BH, Winzeler EA. (2017) Selective Whole-Genome Amplification Is a Robust Method That Enables Scalable Whole-Genome Sequencing of Plasmodium vivax from Unprocessed Clinical Samples. MBio e02257-16.
• Kobylinski KC, Escobedo-Vargas KS, López-Sifuentes VM, Durand S, Smith ES, Baldeviano GC, Gerbasi RV, Ballard SB, Stoops CA, Vásquez GM. (2017) Ivermectin susceptibility, sporontocidal effect, and inhibition of time to re-feed in the Amazonian malaria vector Anopheles darlingi. Malaria J. 16(1):474.

2015

• Alajem A, Biran A, Harikumar A, Sailaja BS, Aaronson Y, Livyatan I, Nissim-Rafinia M, Sommer AG, Mostoslavsky G, Gerbasi VR, Golden DE, Datta A, Sze SK, Meshorer E. (2015). Differential association of chromatin proteins identifies BAF60a/SMARCD1 as a regulator of embryonic stem cell differentiation. Cell Rep (12):2019-31.
• Valdivia HO, Reis-Cunha JL, Rodriguez-Luiz GF, Baptista RP, Baldeviano GC, Gerbasi RV, Dobson DE, Pratlong F, Bastien P, Lescano AG, Beverley SM, Batholomeu DC (2015) Comparative Genomic Analysis of Leishmania (Viannia) peruviana and Leishmania (Viannia) braziliensis. BMC Genomics (16): 715.

2013

• McCoy, M.E., Golden, H.E., Doll, T.A., Yang, Y., Kaba, S.A., Zou, X., Gerbasi, V.R., Burkhard, P., and Lanar, D.E. (2013) Mechanisms of protective immune responses induced by the Plasmodium falciparum circumsporozoite protein-based, self-assembling protein nanoparticle vaccine. Malaria Journal (1): 136.
• Zou, X., House, BL., Zyzak, MD., Richie, TL., Gerbasi V.R. (2013) Towards an Optimized Inhibition of Liver Stage Development Assay (ILSDA) for Plasmodium falciparum. Malaria Journal (1): 394.

2012

• Siedman, D., Johnson, D., Gerbasi V., Golden D., Orlando, R., Hajduk, S. (2012).  A Mitochondrial membrane complex that contains proteins necessary for tRNA import in Trypanosoma brucei. J Biol Chem 287(12):8892-903.

2003-2011

• Gerbasi, V., Lutsenko, S., and Lewis, E. J. (2003). A mutation in the ATP7B copper transporter causes reduced dopamine beta-hydroxylase and norepinephrine in mouse adrenal. Neurochem Res 28, 867-873.
• Rychlik, J. L., Gerbasi, V., and Lewis, E. J. (2003). The interaction between dHAND and Arix at the dopamine beta-hydroxylase promoter region is independent of direct dHAND binding to DNA. J Biol Chem 278, 49652-49660.
• Gerbasi, V. R., Weaver, C. M., Hill, S., Friedman, D. B., and Link, A. J. (2004). Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression. Mol Cell Biol 24, 8276-8287.
• Link, A. J., Fleischer, T. C., Weaver, C. M., Gerbasi, V. R., and Jennings, J. L. (2005). Purifying protein complexes for mass spectrometry: applications to protein translation. Methods 35, 274-290.
• Gerbasi, V. R., and Link, A. J. (2007). The myotonic dystrophy type-2 protein ZNF9 is part of an ITAF complex that promotes cap-independent translation. Molecular Cellular Proteomics 6, 1049-1058.
• Golden, D.E., Gerbasi, V.R., and Sontheimer, E.J. (2008).  An inside job for siRNAs. Mol Cell 31, 309-312.
• Gerbasi, V.R., Golden, D.E., Hurtado, S.L. and Sontheimer, E.J. (2010).  Proteomic identification of Drosophila siRNA-associated factors. Molecular Cellular Proteomics 9, 1866-72.
• Gerbasi, V.R., Preall, J., Golden, D.E., Cummins, T., Powell, D.W., and Sontheimer, E.J.  (2011) Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis.  PNAS 108, 3204-9.