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Fundamental and Computational Sciences Directorate

Staff information


Mary Lipton

Biosystms Dynamics & Simula
Pacific Northwest National Laboratory
PO Box 999
MSIN: K8-98
Richland, WA 99352


Dr. Mary Lipton is currently a Staff Scientist at the Pacific Northwest National Laboratory and Adjunct Professor at Washington State University-Tri-Cities in Richland and Adjunct Professor in the Institute for Biological Chemistry and Washington State University Pullman. She is nationally recognized for her research in microbial proteomics, specifically for developing and applying new mass spectrometry-based technologies for the characterization of environmental microbes and microbial communities.

Research Interests

  • Relative quantitative determination of changes in protein abundance
  • Determination of protein localization and the determination of temporal changes in protein expression patterns
  • These applications have elucidated important biological processes that play integral roles in microbially mediated bioenergy production, bioremediation, and carbon sequestration. Additionally, the comparison of proteomic data across a variety of microorganisms has allowed the determination of proteins that are necessary for life and those that define the lifestyle of a class of microbes. Such insights will have impact on understanding microbial function across the “Tree of Life” as well as in the emerging field of synthetic biology.

Education and Credentials

  • Postdoc., Pacific Northwest National Laboratory, 1993-96
  • Ph.D., Biochemistry, University of South Carolina, 1993
  • B.S., Chemistry, Juniata College, Huntington, PA, 1988

Affiliations and Professional Service

  • Protein Society
  • American Society for Biochemistry and Molecular Biology
  • Radiation Research Society
  • American Chemical Society

Awards and Recognitions

  • University of South Carolina's Distinguished Young Alumnus Award, 2005
  • PNNL Outstanding Performance Award for Funding of the proposal "Environmental Sensing, Metabolic Response, and Regulatory Networks in the Respiratory Versatile Bacterium Shewanella oneidensis MR-1" under DOE-OBER's new Microbial Cell Program
  • PNNL Woman of Achievement, 2001
  • EHI Performance Award, PNNL, 2000
  • EHI Performance Award, PNNL, 1999
  • Outstanding Team Performance Award; PNNL, 1999
  • Radiation Research Student Travel Award, 1995
  • Platform Presentation: International Symposia on Radiation Damage to DNA, 1994
  • Radiation Research Student Travel Award 1994 Travel Award / Invited
  • Lilly Travel Grant Award for the Seventh Symposium of the Protein Society, 1993
  • Bouknight Outstanding Teaching Award, 1992

PNNL Publications


  • Lindemann SR, JM Mobberley, JK Cole, LM Markillie, RC Taylor, EL Huang, WB Chrisler, HS Wiley, MS Lipton, WC Nelson, JK Fredrickson, and MF Romine. 2017. "Predicting Species-Resolved Macronutrient Acquisition during Succession in a Model Phototrophic Biofilm Using an Integrated ‘Omics Approach." Frontiers in Microbiology 8:Article No. 1020.  doi:10.3389/fmicb.2017.01020
  • Booker AE, M Borton, R Daly, S Welch, CD Nicora, DW Hoyt, T Wilson, SO Purvine, R Wolfe, S Sharma, PJ Mouser, DR Cole, MS Lipton, KC Wrighton, and MJ Wilkins. 2017. "Sulfide Generation by Dominant Halanaerobium Microorganisms in Hydraulically Fractured Shales." mSphere 2:Article No. e00257-17.  doi:10.1128/mSphereDirect.00257-17
  • Solden L, DW Hoyt, WB Collins, JE Plank, R Daly, E Hildebrand, TJ Beavers, R Wolfe, CD Nicora, SO Purvine, M Carstensen, MS Lipton, DE Spalinger, JL Firkins, BA Wolfe, and KC Wrighton. 2017. "New roles in hemicellulosic sugar fermentation for the uncultivated Bacteroidetes family BS11." The ISME Journal 11(3):691-703.  doi:10.1038/ismej.2016.150
  • Strand DD, AK Livingston, M Satoh-Cruz, T Koepke, HM Enlow, N Fisher, JE Froehlich, JA Cruz, D Minhas, KK Hixson, K Kohzuma, MS Lipton, A Dhingra, and D Kramer. 2017. "Defects in the Expression of Chloroplast Proteins Leads to H2O2 Accumulation and Activation of Cyclic Electron Flow around Photosystem I." Frontiers in Plant Science 7:2073.  doi:10.3389/fpls.2016.02073
  • Unkefer CJ, RT Sayre, JK Magnuson, DB Anderson, I Baxter, IK Blaby, JK Brown, M Carleton, RA Cattolico, T Dale, TP Devarenne, CM Downes, SK Dutcher, DT Fox, UW Goodenough, J Jaworski, JE Holladay, DM Kramer, AT Koppisch, MS Lipton, BL Marrone, MM Mccormick, I Molnar, JB Mott, KL Ogden, EA Panisko, M Pellegrini, JE Polle, JW Richardson, M Sabarskyo, SR Starkenburg, GD Stormo, M Teshima, S Twary, PJ Unkefer, J Yuan, and JA Olivares. 2017. "Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts." Algal Research 22:187-215.  doi:10.1016/j.algal.2016.06.002


  • Anderson LN, PK Koech, AE Plymale, EV Landorf , A Konopka, F Collart, MS Lipton, MF Romine, and AT Wright. 2016. "Live Cell Discovery of Microbial Vitamin Transport and Enzyme-Cofactor Interactions." ACS Chemical Biology 11(2):345-354.  doi:10.1021/acschembio.5b00918
  • Smith D, CD Nicora, P Carini, MS Lipton, AD Norbeck, RD Smith, and SJ Giovannoni. 2016. "Proteome Remodeling in Response to Sulfur Limitation in “Candidatus Pelagibacter ubique”." mSystems 1(4):e00068-16.  doi:10.1128/mSystems.00068-16
  • Stuart R, X Mayali, AA Boaro, A Zelma, RC Everroad, DJ Nilson, PK Weber, MS Lipton, BM Bebout, J Pett-Ridge, and MP Thelen. 2016. "Light Regimes Shape Utilization of Extracellular Organic C and N in a Cyanobacterial Biofilm." mBio 7(3):Article No. e00650-16.  doi:10.1128/mBio.00650-16
  • Sun J, JD Todd, JC Thrash, Y Qian, MC Qian, B Temperton, J Guo, EMK Fowler, JT Aldrich, CD Nicora, MS Lipton, RD Smith, P De Leenheer, SH Payne, AW Johnston, CL Davie-Martin, KH Halsey, and SJ Giovannoni. 2016. "The abundant marine bacterium Pelagibacter simultaneously catabolizes dimethylsulfoniopropionate to the gases dimethyl sulfide and methanethiol." Nature Microbiology 1(8):Article No. 16065.  doi:10.1038/nmicrobiol.2016.65


  • Aylward FO, L Khadempour, D Tremmel, BR McDonald, CD Nicora, S Wu, RJ Moore, DJ Orton, ME Monroe, PD Piehowski, SO Purvine, RD Smith, MS Lipton, KE Burnum-Johnson, and CR Currie. 2015. "Enrichment and Broad Representation of Plant Biomass-Degrading Enzymes in the Specialized Hyphal Swellings of Leucoagaricus gongylophorus, the Fungal Symbiont of Leaf-Cutter Ants." PLoS One 10(8):e0134752.  doi:10.1371/journal.pone.0134752
  • Burnet MC, A Dohnalkova, AP Neumann, MS Lipton, RD Smith, G Suen, and SJ Callister. 2015. "Evaluating Models of Cellulose Degradation by Fibrobacter succinogenes S85." PLoS One 10(12):e0143809.  doi:10.1371/journal.pone.0143809
  • Merkley ED, KC Wrighton, C Castelle, BJ Anderson, MJ Wilkins, V Shah, T Arbour, JN Brown, SW Singer, RD Smith, and MS Lipton. 2015. "Changes in protein expression across laboratory and field experiments in Geobacter bemidjiensis." Journal of Proteome Research 14(3):1361-1375.  doi:10.1021/pr500983v
  • Payne SH, ME Monroe, CC Overall, GR Kiebel, MG Degan, BC Gibbons, GM Fujimoto, SO Purvine, JN Adkins, MS Lipton, and RD Smith. 2015. "The Pacific Northwest National Laboratory library of bacterial and archaeal proteomic biodiversity." Scientific Data 2:Art. No. 150041.  doi:10.1038/sdata.2015.41


  • Slysz GW, LA Steinke, DM Ward, CG Klatt, TRW Clauss, SO Purvine, SH Payne, GA Anderson, RD Smith, and MS Lipton. 2014. "Automated data extraction from in situ protein stable isotope probing studies." Journal of Proteome Research 13(3):1200-1210.  doi:10.1021/pr400633j
  • Cao L, N Tolic, Y Qu, D Meng, R Zhao, Q Zhang, RJ Moore, EM Zink, MS Lipton, L Pasa-Tolic, and S Wu. 2014. "Characterization of intact N- and O-linked glycopeptides using higher energy collisional dissociation." Analytical Biochemistry 452:96-102.  doi:10.1016/j.ab.2014.01.003
  • Huang EL, FO Aylward, YM Kim, BJM Webb-Robertson, CD Nicora, Z Hu, TO Metz, MS Lipton, RD Smith, CR Currie, and KE Burnum-Johnson. 2014. "The fungus gardens of leaf-cutter ants undergo a distinct physiological transition during biomass degradation." Environmental Microbiology Reports 6(4):389-95.  doi:10.1111/1758-2229.12163
  • Keating DH, Y Zhang, IM Ong, S Mcllwain, EH Morales, JA Grass, M Tremaine, W Bothfeld, A Higbee, A Ulbrich, AJ Balloon, MS Westphall, JT Aldrich, MS Lipton, J Kim, OV Moskvin, YV Bukhman, JJ Coon, PJ Kiley, DM Bates, and R Landick. 2014. "Aromatic inhibitors derived from ammonia-pretreated lignocellulose hinder bacterial ethanologenesis by activating regulatory circuits controlling inhibitor efflux and detoxification." Frontiers in Microbiology 5:Article No. 402.  doi:10.3389/fmicb.2014.00402
  • Qu Y, J Feng, S Deng, L Cao, Q Zhang, R Zhao, Z Zhang, Y Jiang, EM Zink, SE Baker, MS Lipton, L Pasa-Tolic, JZ Hu, and S Wu. 2014. "Structural Analysis of N- and O-glycans Using ZIC-HILIC/Dialysis Coupled to NMR Detection." Fungal Genetics and Biology : FG & B 72:207-215.  doi:10.1016/j.fgb.2014.08.001
  • Wrighton KC, C Castelle, MJ Wilkins, LA Hug, I Sharon, BC Thomas, KM Handley, SW Mullin, CD Nicora, A Singh, MS Lipton, PE Long, KH Williams, and JF Banfield. 2014. "Metabolic interdependencies between phylogenetically novel fermenters and respiratory organisms in an unconfined aquifer." The ISME Journal 8(7):1452-1463.  doi:10.1038/ismej.2013.249


  • Aylward FO, KE Burnum-Johnson, SG Tringe, C Teiling, D Tremmel, J Moeller, JJ Scott, KW Barry, PD Piehowski, CD Nicora, S Malfatti, ME Monroe, SO Purvine, LA Goodwin, RD Smith, G Weinstock, N Gerardo, G Suen, MS Lipton, and CR Currie. 2013. "Leucoagaricus gongylophorus Produces Diverse Enzymes for the Degradation of Recalcitrant Plant Polymers in Leaf-Cutter Ant Fungus Gardens." Applied and Environmental Microbiology 79(12):3770-3778.  doi:10.1128/AEM.03833-12
  • Nicora CD, BJ Anderson, SJ Callister, AD Norbeck, SO Purvine, JK Jansson, OU Mason, M David, DD Jurelevicius, RD Smith, and MS Lipton. 2013. "Amino acid treatment enhances protein recovery from sediment and soils for metaproteomic studies ." Proteomics 13(18-19):2776-2785.  doi:10.1002/pmic.201300003
  • Qu Y, S Wu, R Zhao, EM Zink, DJ Orton, RJ Moore, D Meng, TRW Clauss, JT Aldrich, MS Lipton, and L Pasa-Tolic. 2013. "Automated Immobilized Metal Affinity Chromatography System for Enrichment of Escherichia coli Phosphoproteome." Electrophoresis 34(11):1619-1626.  doi:10.1002/elps.201200628
  • Taylor RC, BJM Webb-Robertson, LM Markillie, MH Serres, BE Linggi, JT Aldrich, EA Hill, MF Romine, MS Lipton, and HS Wiley. 2013. "Changes in Translational Efficiency is a Dominant Regulatory Mechanism in the Environmental Response of Bacteria." Integrative Biology 5(11):1393-1406.  doi:10.1039/C3IB40120K
  • Wilkins MJ, KC Wrighton, CD Nicora, KH Williams, LA McCue, KM Handley, CS Miller, L Giloteaux, AP Montgomery, DR Lovley, JF Banfield, PE Long, and MS Lipton. 2013. "Fluctuations in Species-Level Protein Expression Occur during Element and Nutrient Cycling in the Subsurface ." PLoS One 8(3):e57819.  doi:10.1371/journal.pone.0057819
  • Wu S, RN Brown, SH Payne, D Meng, R Zhao, N Tolic, L Cao, AK Shukla, ME Monroe, RJ Moore, MS Lipton, and L Pasa-Tolic. 2013. "Top-down characterization of the post-translationally modified intact periplasmic proteome of the bacterium Novosphingobium aromaticivorans ." International Journal of Proteomics 2013:279590.  doi:10.1155/2013/279590


  • Aylward FO, KE Burnum, JJ Scott, G Suen, SG Tringe, SM Adams, KW Barry, CD Nicora, PD Piehowski, SO Purvine, GJ Starrett, LA Goodwin, RD Smith, MS Lipton, and CR Currie. 2012. "Metagenomic and metaproteomic insights into bacterial communities in leaf-cutter ant fungus gardens." The ISME Journal 6(9):1688-1701.  doi:10.1038/ismej.2012.10
  • Cao L, DA Bryant, AA Schepmoes, K Vogl, RD Smith, MS Lipton, and SJ Callister. 2012. "Comparison of Chloroflexus aurantiacus strain J-10-fl proteomes of cells grown chemoheterotrophically and photoheterotrophically." Photosynthesis Research 110(3):153-168.  doi:10.1007/s11120-011-9711-8
  • Fang Y, MJ Wilkins, SB Yabusaki, MS Lipton, and PE Long. 2012. "Evaluation of a Genome-Scale In Silico Metabolic Model for Geobacter metallireducens Using Proteomic Data from a Field Biostimulation Experiment." Applied and Environmental Microbiology 78(24):8735 - 8742.  doi:10.1128/AEM.01795-12
  • Liu J, Z Wang, SM Belchik, M Edwards, C Liu, DW Kennedy, ED Merkley, MS Lipton, JN Butt, D Richardson, JM Zachara, JK Fredrickson, KM Rosso, and L Shi. 2012. "Identification and Characterization of MtoA: a Decaheme c-Type Cytochrome of the Neutrophilic Fe(II)-oxidizing Bacterium Sideroxydans lithotrophicus ES-1." Frontiers in Microbiological Chemistry 3:Article No. 37.  doi:10.3389/fmicb.2012.00037
  • Merkley ED, BJ Anderson, JH Park, SM Belchik, L Shi, ME Monroe, RD Smith, and MS Lipton. 2012. "Detection and Identification of Heme c-Modified Peptides by Histidine Affinity Chromatography, High-Performance Liquid Chromatography-Mass Spectrometry, and Database Searching." Journal of Proteome Research 11(12):6147-6158.  doi:10.1021/pr3007914
  • Vu T, S Stolyar, GE Pinchuk, EA Hill, LA Kucek, RN Brown, MS Lipton, AL Osterman, JK Fredrickson, A Konopka, AS Beliaev, and JL Reed. 2012. "Genome-scale modeling of light-driven reductant partitioning and carbon fluxes in diazotrophic unicellular cyanobacterium Cyanothece sp. ATCC 51142." PLoS Computational Biology 8(4):e1002460.  doi:10.1371/journal.pcbi.1002460
  • Vu T, S Stolyar, GE Pinchuk, EA Hill, LA Kucek, RN Brown, MS Lipton, AL Osterman, JK Fredrickson, A Konopka, AS Beliaev, and JL Reed. 2012. "Genome-Scale Modeling of Light-Driven Reductant Partitioning and Carbon Fluxes in Diazotrophic Unicellular Cyanobacterium Cyanothece sp. ATCC 51142." PLoS Computational Biology 8(4):E1002460.  doi:10.1371/journal.pcbi.1002460
  • Wrighton KC, BC Thomas, I Sharon, CS Miller, C Castelle, NC VerBerkmoes, MJ Wilkins, RL Hettich, MS Lipton, KH Williams, PE Long, and JF Banfield. 2012. "Fermentation, Hydrogen, and Sulfur Metabolism in Multiple Uncultivated Bacterial Phyla." Science 337(6102):1661-1665.  doi:10.1126/science.1224041
  • Yuan J, B Wei, MS Lipton, and H Gao. 2012. "Impact of ArcA loss in Shewanella oneidensis revealed by comparative proteomics under aerobic and anaerobic conditions." Proteomics 12(12):1957-1969.  doi:10.1002/pmic.201100651


  • Cannon WR, MM Rawlins, DJ Baxter, SJ Callister, MS Lipton, and DA Bryant. 2011. "Large Improvements in MS/MS Based Peptide Identification Rates using a Hybrid Analysis." Journal of Proteome Research 10(5):2306-2317.  doi:10.1021/pr101130b
  • Adams LG, S Khare, SD Lawhon, CA Rossetti, HA Lewin, MS Lipton, JE Turse, DC Wylie, Y Bai, and KL Drake. 2011. "Enhancing the role of veterinary vaccines reducing zoonotic diseases of humans: Linking systems biology with vaccine development." Vaccine 29(41):7197-7206.  doi:10.1016/j.vaccine.2011.05.080
  • Britos LC, E Abeliuk, T Taverner, MS Lipton, H McAdams, and L Shapiro. 2011. "Regulatory Response to Carbon Starvation in Caulobacter crescentus." PLoS One 6(4):e18179.  doi:10.1371/journal.pone.0018179
  • Burnum KE, SJ Callister, CD Nicora, SO Purvine, P Hugenholtz, F Warnecke, RH Scheffrahn, RD Smith, and MS Lipton. 2011. "Proteome Insights into the Symbiotic Relationship Between a Captive Colony of Nasutitermes corniger and its Hindgut Microbiome." The ISME Journal 5(1):161-164.  doi:10.1038/ismej.2010.97
  • Cao B, L Shi, RN Brown, Y Xiong, JK Fredrickson, MF Romine, MJ Marshall, MS Lipton, and H Beyenal. 2011. "Extracellular Polymeric Substances from Shewanella sp. HRCR-1 Biofilms: Characterization by Infrared Spectroscopy and Proteomics." Environmental Microbiology 13(4):1018-1031.  doi:10.1111/j.1462-2920.2010.02407.x
  • Chundawat SP, MS Lipton, SO Purvine, N Uppugundla, D Gao, V Balan, and BE Dale. 2011. "Proteomics based compositional analysis of complex cellulase-hemicellulase mixtures." Journal of Proteome Research 10(10):4365-4372.  doi:10.1021/pr101234z
  • Lennen RM, MA Kruziki, K Kumar, RA Zinkel, KE Burnum, MS Lipton, SW Hoover, DR Ranatunga, TM Wittkopp, WD Marner II, and BF Pfleger. 2011. "Membrane Stresses Induced by Overproduction of Free Fatty Acids in Escherichia coli." Applied and Environmental Microbiology 77(22):8114-8128.  doi:10.1128/AEM.05421-11
  • Polacco BJ, SO Purvine, EM Zink, SP LaVoie, MS Lipton, AO Summers, and SM Miller. 2011. "Discovering Mercury Protein Modifications in Whole Proteomes Using Natural Isotope Distributions Observed in Liquid Chromatography-Tandem Mass Spectrometry." Molecular & Cellular Proteomics. MCP 10(8):Art. No. 10: M110.004853.  doi:10.1074/mcp.M110.004853
  • Wilkins MJ, SJ Callister, M Miletto, KH Williams, CD Nicora, DR Lovely, PE Long, and MS Lipton. 2011. "Development of a biomarker for Geobacter activity and strain composition; Proteogenomic analysis of the citrate synthase protein during bioremediation of U(VI)." Microbial Biotechnology 4(1):55-63.  doi:10.1111/j.1751-7915.2010.00194.x
  • Zhang H, F Yang, W Qian, RN Brown, Y Wang, ED Merkley, JH Park, ME Monroe, SO Purvine, RJ Moore, L Shi, JK Fredrickson, L Pasa-Tolic, RD Smith, and MS Lipton. 2011. "Identification of c-Type Heme-Containing Peptides Using Non-Activated Immobilized Metal Affinity Cchromatography Resin Enrichment and Higher-Energy Collisional Dissociation." Analytical Chemistry 83(19):7260-7268.  doi:10.1021/ac2000829


  • Brown RN, MF Romine, AA Schepmoes, RD Smith, and MS Lipton. 2010. "Mapping the Subcellular Proteome of Shewanella oneidensis MR-1 using Sarkosyl-based fractionation and LC-MS/MS protein identification." Journal of Proteome Research 9(9):4454-4463.  doi:10.1021/pr100215h
  • Callister SJ, MJ Wilkins, CD Nicora, KH Williams, JF Banfield, N VerBerkmoes, RL Hettich, AL N'Guessan, P Mouser, H Elifantz, RD Smith, DR Lovley, MS Lipton, and PE Long. 2010. "Analysis of Biostimulated Microbial Communities from Two Field Experiments Reveals Temporal and Spatial Differences in Proteome Profiles." Environmental Science & Technology 44(23):8897-8903.  doi:10.1021/es101029f
  • DiDonato RJ, ND Young, J Butler, KJ Chin, KK Hixson, P Mouser, MS Lipton, R DeBoy, and B Methe. 2010. "Genome Sequence of the Deltaproteobacterial Strain NaphS2 and Analysis of Differential Gene Expression during Anaerobic Growth on Naphthalene." PLoS One 5(11):Article No. e14072.  doi:10.1371/journal.pone.0014072
  • Mercer R, SJ Callister, MS Lipton, L Pasa-Tolic, H Strnad, V Paces, JT Beatty, and AS Lang. 2010. "Loss of the Response Regulator CtrA Causes Pleiotropic Effects on Gene Expression but Does Not Affect Growth Phase Regulation in Rhodobacter capsulatus." Journal of Bacteriology 192(11):2701-2710.  doi:10.1128/JB.00160-10
  • Schutzer SE, T Liu, B Natelson, TE Angel, AA Schepmoes, SO Purvine, KK Hixson, MS Lipton, DG Camp, II, PK Coyle, RD Smith, and J Bergquist. 2010. "Establishing the Proteome of Normal Human Cerebrospinal Fluid ." PLoS One 5(6):e10980-.  doi:10.1371/journal.pone.0010980
  • Smith DP, JB Kitner, AD Norbeck, TRW Clauss, MS Lipton, MS Schwalbach, L Steindler, CD Nicora, RD Smith, and SJ Giovannoni. 2010. "Transcriptional and translational regulatory responses to iron limitation in the globally distributed marine bacterium Candidatus Pelagibacter ubique." PLoS One 5(5):Article Number: e10487. 
  • Turse JE, MJ Marshall, JK Fredrickson, MS Lipton, and SJ Callister. 2010. "An empirical strategy for characterizing bacterial proteomes across species in the absence of genomic sequences." PLoS One 5(11):Article No.: e13968.  doi:10.1371/journal.pone.0013968
  • Zhang H, RN Brown, W Qian, ME Monroe, SO Purvine, RJ Moore, MA Gritsenko, L Shi, MF Romine, JK Fredrickson, L Pasa-Tolic, RD Smith, and MS Lipton. 2010. "Quantitative analysis of cell surface membrane proteins using membrane-impermeable chemical probe coupled with 18O labeling." Journal of Proteome Research 9(5):2160-2169. 


  • Henne KL, JE Turse, CD Nicora, MS Lipton, SL Tollaksen, C Lindberg, G Babbnig, CS Giometti, CN Nakatsu, DK Thompson, and A Konopka. 2009. "Global Proteomic Analysis of the Chromate Response in Arthrobacter sp strain FB24." Journal of Proteome Research 8(4)(Sp.Iss.SI):1704-16.  doi:10.1021/Pr800705f
  • Kim W, MW Silby, SO Purvine, JS Nicoll, KK Hixson, ME Monroe, CD Nicora, MS Lipton, and SB Levy. 2009. "Proteomic Detection of Non-Annotated Protein-Coding Genes in Pseudomonas fluorescens Pf0-1." PLoS One 4(12):Article Number: e8455.  doi:10.1371/journal.pone.0008455
  • Konstantinidis K, MH Serres, MF Romine, JLM Rodrigues, JM Auchutung, LA McCue, MS Lipton, A Obraztsova, CS Giometti, KH Nealson, JK Fredrickson, and JM Tiedje. 2009. "Comparative systems biology across an evolutionary gradient within the Shewanella genus ." Proceedings of the National Academy of Sciences of the United States of America 106(37):15909-15914.  doi:10.1073/pnas.0902000106
  • Sowell SM, L Wilhelm, AD Norbeck, MS Lipton, CD Nicora, DF Barofsky, C carlson, RD Smith, and SJ Giovannoni. 2009. " Transport Functions Dominate the SAR11 Metaproteome at Low-Nutrient Extremes in the Sargasso Sea." The ISME Journal 3(1):93-105. 
  • Wilkins MJ, NC VerBerkmoes, KH Williams, SJ Callister, P Mouser, H Elifantz, AL N'Guessan, BC Thomas, CD Nicora, MB Shah, P Abraham, MS Lipton, DR Lovely, RL Hettich, PE Long, and JF Banfield. 2009. "Proteogenomic monitoring of Geobacter physiology during stimulated uranium bioremediation." Applied and Environmental Microbiology 75(20):6591-6599.  doi:10.1128/AEM.01064-09


  • Ansong C, SO Purvine, JN Adkins, MS Lipton, and RD Smith. 2008. "Proteogenomics: the needs and roles to be filled by proteomics in genome annotation." Briefings in Functional Genomics and Proteomics 7(1):50-62. 
  • Callister SJ, LA McCue, JE Turse, ME Monroe, KJ Auberry, RD Smith, JN Adkins, and MS Lipton. 2008. "Comparative Bacterial Proteomics: Analysis of the Core Genome Concept." PLoS One 3(2):e1542.  doi:10.1371/journal.pone.0001542
  • Ding YHR, KK Hixson, M Aklujkar, MS Lipton, RD Smith, DR Lovley, and T Mester. 2008. "Proteome of Geobacter sulfurreducens grown with Fe(III) oxide or Fe(III) citrate as the electron acceptor." Biochimica et Biophysica Acta--Proteins and Proteomics 1784(12):1935-1941.  doi:10.1016/j.bbapap.2008.06.011
  • Du X, SJ Callister, NP Manes, JN Adkins, RA Alexandridis, X Zeng, JH Roh, WE Smith, TJ Donohue, S Kaplan, RD Smith, and MS Lipton. 2008. "A Computational Strategy to Analyze Label-Free Temporal Bottom-up Proteomics Data." Journal of Proteome Research 7(7):2595-2604.  doi:10.1021/pr0704837
  • Elias DA, SL Tollaksen, DW Kennedy, HM Mottaz, CS Giometti, JS Mclean, EA Hill, GE Pinchuk, MS Lipton, JK Fredrickson, and YA Gorby. 2008. "The Influence of Cultivation Methods on Shewanella oneidensis Physiology and Proteome Expression ." Archives of Microbiology 189(4):313-324.  doi:10.1007/s00203-007-0321-y
  • Eraso JM, JH Roh, X Zeng, SJ Callister, MS Lipton, and S Kaplan. 2008. "Role of the global transcriptional regulator PrrA in Rhodobacter sphaeroides 2.4.1: combined transcriptome and proteome analysis." Journal of Bacteriology 190(14):4831-4848.  doi:10.1128/JB.00301-08
  • Gupta N, J Benhamida, V Bhargava, D Goodman, E Kain , I Kerman, N Nguyen , N Ollikainen, J Rodriguez, J Wang, MS Lipton, MF Romine, V Bafna, RD Smith, and PA Pevzner. 2008. "Comparative proteogenomics: combining mass spectrometry and comparative genomics to analyze multiple genomes." Genome Research 18(7):1133-1142. 
  • Romine MF, T Carlson, AD Norbeck, LA McCue, and MS Lipton. 2008. "Identification of Mobile Elements and Pseudogenes in the Shewanella oneidensis MR-1 Genome." Applied and Environmental Microbiology 74(10):3257-3265. 
  • Sowell SM, AD Norbeck, MS Lipton, CD Nicora, SJ Callister, RD Smith, DF Barofsky, and SJ Giovannoni. 2008. "Proteomic Analysis of Stationary Phase in the Marine Bacterium 'Candidatus Pelagibacter ubique'." Applied and Environmental Microbiology 74(13):4091-4100.  doi:10.1128/AEM.00599-08
  • Sowell SM, AD Norbeck, MS Lipton, CD Nicora, SJ Callister, RD Smith, DF Barofsky, and SJ Giovannoni. 2008. "Proteomic Analysis of Stationary Phase in the Marine Bacterium 'Candidatus Pelagibacter ubique'." Applied and Environmental Microbiology 74(13):4091-4100.  doi:10.1128/AEM.00599-08
  • Webb-Robertson BJM, WR Cannon, CS Oehmen, AR Shah, V Gurumoorthi, MS Lipton, and KM Waters. 2008. "A Support Vector Machine model for the prediction of proteotypic peptides for accurate mass and time proteomics." Bioinformatics 24(13):1503-1509.  doi:10.1093/bioinformatics/btn218


  • Elias DA, F Yang, HM Mottaz, AS Beliaev, and MS Lipton. 2007. "Enrichment of Functional Redox Reactive Proteins and Identification by Mass Spectrometry Results in Several Terminal Fe(III)-reducing Candidate Proteins in Shewanella oneidensis MR-1." Journal of Microbiological Methods 68(2):367-375. 
  • Gupta N, S Tanner, N Jaitly, JN Adkins, MS Lipton, R Edwards, MF Romine, A Osterman, V Bafna, RD Smith, and PA Pevzner. 2007. "Whole proteome analysis of post-translational modifications: applications of mass-spectrometry for proteogenomic annotation." Genome Research 17(9):1362-1377.  doi:0.1101/gr.6427907
  • Resch W, KK Hixson, RJ Moore, MS Lipton, and B Moss. 2007. "Protein Composition of the Vaccinia Virus Mature Virion." Virology 358(1):233-247. 
  • Yang F, N Jaitly, H Jayachandran, Q Lou, ME Monroe, X Du, MA Gritsenko, R Zhang, DJ Anderson, SO Purvine, JN Adkins, RJ Moore, HM Mottaz, SJ Ding, MS Lipton, DG Camp, II, HR Udseth, RD Smith, and SS Rossie. 2007. "Applying a Targeted Label-free Approach using LC-MS AMT Tags to Evaluate Changes in Protein Phosphorylation Following Phosphatase Inhibition." Journal of Proteome Research 6(11):4489-4497.  doi:10.1021/pr070068e
  • Zeng X, JH Roh, SJ Callister, C Tavano, T Donohue, MS Lipton, and S Kaplan. 2007. "Proteomic characterization of the Rhodobacter sphaeroides 2.4.1 photosynthetic membrane: Identification of New Proteins." Journal of Bacteriology 189(20):7464-7474.  doi:10.1128/JB.00946-07


  • Norbeck AD, SJ Callister, ME Monroe, N Jaitly, DA Elias, MS Lipton, and RD Smith. 2006. "Proteomic approaches to bacterial differentiation ." Journal of Microbiological Methods 67(3):473-486.  doi:10.1016/j.mimet.2006.04.024
  • Norbeck AD, SJ Callister, ME Monroe, N Jaitly, DA Elias, MS Lipton, and RD Smith. 2006. "Proteomic approaches to bacterial differentiation." Journal of Microbiological Methods 67(3):473-486. 
  • Callister SJ, CD Nicora, X Zeng, JH Roh, M Dominguez, C Tavano, ME Monroe, S Kaplan, T Donohue, RD Smith, and MS Lipton. 2006. "Comparison of aerobic and photosynthetic Rhodobacter sphaeroides 2.4.1 proteomes." Journal of Microbiological Methods 67(3):424-436.  doi:10.1016/j.mimet.2006.04.021
  • Callister SJ, M Dominguez, CD Nicora, X Zeng, C Tavano, S Kaplan, T Donohue, RD Smith, and MS Lipton. 2006. "Application of the Accurate Mass and Time Tag Approach to the Proteome Analysis of Sub-cellular Fractions Obtained from Rhodobacter sphaeroides 2.4.1 Aerobic and Photosynthetic Cell Cultures." Journal of Proteome Research 5(8):1940-1947. 
  • Callister SJ, RC Barry, JN Adkins, ET Johnson, W Qian, BJM Webb-Robertson, RD Smith, and MS Lipton. 2006. "Normalization Approaches for Removing Systematic Biases Associated with Mass Spectrometry and Label-Free Proteomics." Journal of Proteome Research 5(2):277-286.  doi:10.1021/pr050300l
  • Ding YHR, KK Hixson, CS Giometti, A Stanley, A Esteve-Nunez, T Khare, SL Tollaksen, W Zhu, JN Adkins, MS Lipton, RD Smith, T Mester, and DR Lovley. 2006. "The Proteome of Dissimilatory Metal-reducing Microorganism Geobacter Sulfurreducens under Various Growth Conditions." Biochimica et Biophysica Acta--Proteins and Proteomics 1764(7):1198-1206.  doi:10.1016/j.bbapap.2006.04.017
  • Elias DA, ME Monroe, RD Smith, JK Fredrickson, and MS Lipton. 2006. "Confirmation of the Expression of a Large Set of Conserved Hypothetical Proteins in Shewanella oneidensis MR-1." Journal of Microbiological Methods 66(2):223-233. 
  • Fang R, DA Elias, ME Monroe, Y Shen, M McIntosh, P Wang, CD Goddard, SJ Callister, RJ Moore, YA Gorby, JN Adkins, JK Fredrickson, MS Lipton, and RD Smith. 2006. "Differential Label-free Quantitative Proteomic Analysis of Shewanella oneidensis Cultured under Aerobic and Suboxic Conditions by Accurate Mass and Time Tag Approach." Molecular & Cellular Proteomics. MCP 5(4):714-725.  doi:10.1074/mcp.M500301-MCP200
  • Hixson KK, JN Adkins, SE Baker, RJ Moore, RD Smith, SL McCutchen-Maloney, and MS Lipton. 2006. "Biomarker Candidate Identification in Yersinia Pestis Using Organism-Wide Semiquantitative Proteomics ." Journal of Proteome Research 5(11):3008-3017. 
  • Lipton MS, MF Romine, ME Monroe, DA Elias, L Pasa-Tolic, GA Anderson, DJ Anderson, JK Fredrickson, KK Hixson, CD Masselon, HM Mottaz, N Tolic, and RD Smith. 2006. "AMT Tag Approach to Proteomic Characterization of Deinococcus Radiodurans and Shewanella Oneidensis ." In Microbial Proteomics, Methods of Biochemical Analysis, vol. 49, ed. I. Humphery-Smith and M. Hecker, pp. 113-134.  John Wiley & Sons, Inc., Hoboken, NJ. 
  • Petritis K, LJ Kangas, B Yan, ME Monroe, EF Strittmatter, W Qian, JN Adkins, RJ Moore, Y Xu, MS Lipton, DG Camp, II, and RD Smith. 2006. "Improved peptide elution time prediction for reversed-phase liquid chromatography-MS by incorporating peptide sequence information." Analytical Chemistry 78(14):5026-5039.  doi:10.1021/ac060143p
  • Simpson DC, S Ahn, L Pasa-Tolic, B Bogdanov, HM Mottaz, AN Vilkov, GA Anderson, MS Lipton, and RD Smith. 2006. "Using size exclusion chromatography-RPLC and RPLC-CIEF as two-dimensional separation strategies for protein profiling." Electrophoresis 27(13):2722-2733.  doi:10.1002/elps.200600037
  • Simson DC, S Ahn, L Pasa-Tolic, B Bogdanov, HM Brewer, AN Vilkov, GA Anderson, MS Lipton, and RD Smith. 2006. "Using size exclusion chromatography-RPLC and RPLC-CIEF as two-dimensional separation strategies for protein profiling ." Electrophoresis 27(13):2722-2733.  doi:10.1002/elps.200600037
  • Yang F, DL Stenoien, EF Strittmatter, JH Wang, L Ding, MS Lipton, ME Monroe, CD Nicora, MA Gritsenko, K Tang, R Fang, JN Adkins, DG Camp, II, DJ Chen, and RD Smith. 2006. "Phosphoproteome Profiling of Human Skin Fibroplast Cells in Response to Low- and High-Dose Irradiation ." Journal of Proteome Research 5(5):1252-1260. 


  • Elias DA, ME Monroe, MJ Marshall, MF Romine, AS Beliaev, JK Fredrickson, GA Anderson, RD Smith, and MS Lipton. 2005. "Global detection and characterization of hypothetical proteins in Shewanella oneidensis MR-1 using LC-MS based proteomics based proteomics." Proteomics 5(12):3120-3130.  doi:10.1002/pmic.200401140
  • Havre SL, M Singhal, DA Payne, MS Lipton, and BJM Webb-Robertson. 2005. "Enabling Proteomics Discovery Through Visual Analysis." IEEE Engineering in Medicine and Biology Magazine 24(3):50-57. 
  • Huang Y, JM Triscari, GC Tseng, L Pasa-Tolic, MS Lipton, RD Smith, and VH Wysocki. 2005. "Statistical Characterization of the Charge State and Residue Dependence of Low-Energy CID Peptide Dissociation Patterns." Analytical Chemistry 77(18):5800-5813. 
  • Kolker E, AF Picone, MY Galperin, MF Romine, R Higdon, KS Makarova, N Kolker, GA Anderson, X Qiu, KJ Auberry, G Babnigg, AS Beliaev, P Edlefsen, DA Elias, YA Gorby, T Holzman, J Klappenbach, K Konstantinidis, ML Land, MS Lipton, LA McCue, ME Monroe, L Pasa-Tolic, GE Pinchuk, SO Purvine, M Serres, S Tsapin, BA Zakrajsek, W Zhu, J Zhou, F Larimer, C Lawrence, M Riley, FR Collart, JR Yates, III, RD Smith, CS Giometti, K Nealson, JK Fredrickson, and JM Tiedje. 2005. "Global Profiling of Shewanella oneidensis MR-1: Expression of Hypothetical Genes and Improved functional annotations." Proceedings of the National Academy of Sciences of the United States of America 102(6):2099-2104. 
  • Masselon CD, L Pasa-Tolic, N Tolic, GA Anderson, B Bogdanov, AN Vilkov, Y Shen, R Zhao, W Qian, MS Lipton, DG Camp, II, and RD Smith. 2005. "Targeted Comparative Proteomics by Liquid Chromatography - Tandem Fourier ion cyclotron resonance Mass Spectrometry." Analytical Chemistry 77(2):400-406. 
  • Schad M, MS Lipton, P Giavalisco, RD Smith, and J Kehr. 2005. "Evaluation of two-dimensional electrophoresis and liquid chromatography - tandem mass spectrometry for tissue-specific protein profiling of laser-microdissected plant samples." Electrophoresis 26(14):2729-2738. 
  • Schmid AK, MS Lipton, HM Mottaz, ME Monroe, RD Smith, and ME Lidstrom. 2005. "Global whole-cell FTICR mass spectrometric proteomics analysis of the heat shock response in the radioresistant bacterium Deinococcus radiodurans." Journal of Proteome Research 4(3):709-718. 
  • Yang F, B Bogdanov, EF Strittmatter, AN Vilkov, MA Gritsenko, L Shi, DA Elias, S Ni, MF Romine, L Pasa-Tolic, MS Lipton, and RD Smith. 2005. "Characterization of purified c-type heme-containing peptides and identification of c-type heme-attachment sites in Shewanella oneidenis cytochromes using mass spectrometry." Journal of Proteome Research 4(3):846-854.  doi:10.1021/pr0497475


  • Shen Y, N Tolic, CD Masselon, L Pasa-Tolic, DG Camp, II, GA Anderson, RD Smith, and MS Lipton. 2004. "Nanoscale Proteomics." Analytical and Bioanalytical Chemistry 378(4):1037-1045. 
  • Huang Y, JM Triscari, L Pasa-Tolic, GA Anderson, MS Lipton, RD Smith, and VH Wysocki. 2004. "Dissociation Behavior of Doubly-Charged Tryptic Peptides: Correlation of Gas-Phase Cleavage Abundance with Ramachandran Plots." Journal of the American Chemical Society 126(10):3034-3035. 
  • Romine MF, DA Elias, ME Monroe, KJ Auberry, R Fang, JK Fredrickson, GA Anderson, RD Smith, and MS Lipton. 2004. "Validation of Shewanella oneidensis MR-1 Small Proteins by AMT Tag-based Proteome Analysis." OMICS. A Journal of Integrative Biology 8(3):239-254. 


  • Petritis K, LJ Kangas, PL Ferguson, GA Anderson, L Pasa-Tolic, MS Lipton, KJ Auberry, EF Strittmatter, Y Shen, R Zhao, and RD Smith. 2003. "Use of artificial neural networks for the accurate prediction of peptide liquid chromatography elution times in proteome analyses." Analytical Chemistry 75(5):1039-1048. 


  • Hixson KK, N Rodriguez, DG Camp, II, EF Strittmatter, MS Lipton, and RD Smith. 2002. "Evaluation of Enzymatic Digestion and Liquid Chromatography-Mass Spectrometry Peptide Mapping of the Integral Membrane Protein Bacteriorhodopsin." Electrophoresis 23(18):3224-3232. 
  • Blonder J, MB Goshe, RJ Moore, L Pasa-Tolic, CD Masselon, MS Lipton, and RD Smith. 2002. "Enrichment of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry." Journal of Proteome Research 1(4):351-360. 
  • Lipton MS, L Pasa-Tolic, GA Anderson, DJ Anderson, DL Auberry, JR Battista, MJ Daly, JK Fredrickson, KK Hixson, HM Kostandarithes, CD Masselon, LM Markillie, RJ Moore, MF Romine, Y Shen, EF Strittmatter, N Tolic, HR Udseth, A Venkateswaran, KK Wong, R Zhao, and RD Smith. 2002. "Global Analysis of Deinococcus Radiodurans Proteome by Csing Accurate Mass Tags." Proceedings of the National Academy of Sciences of the United States of America 99(17):11049-11054. 
  • Luo H, MS Lipton, and RD Smith. 2002. "Charge Effects for Differentiation of Oligodeoxynucleotide Isomers Containing 8-oxo-dG Residues." Journal of the American Society for Mass Spectrometry 13:195-199. 
  • Pasa-Tolic L, MS Lipton, CD Masselon, GA Anderson, Y Shen, N Tolic, and RD Smith. 2002. "Gene expression profiling using advanced mass spectrometric approaches." Journal of Mass Spectrometry 37(12):1185-1198. 
  • Smith RD, GA Anderson, MS Lipton, CD Masselon, L Pasa-Tolic, Y Shen, and HR Udseth. 2002. "The Use of Accurate Mass Tags for High-Throughput Microbial Proteomics." OMICS. A Journal of Integrative Biology 6(1):61-90. 
  • Smith RD, GA Anderson, MS Lipton, L Pasa-Tolic, Y Shen, TP Conrads, TD Veenstra, and HR Udseth. 2002. "An Accurate Mass Tag Strategy for Quantitative and High-Throughput Proteome Measurements." Proteomics 2:513-523. 
  • Smith RD, GA Anderson, TP Conrads, CD Masselon, MS Lipton, L Pasa-Tolic, and TD Veenstra. 2002. "Advanced Mass Spectrometric Approaches for Rapid and Quantitative Proteomics." Chapter 8 in Applied Electrospray Mass Spectrometry, ed. BN Pramanik,, AK Ganguly & ML Gross, pp. 307-360.  Marcel Dekker, New York, NY. 


  • Shen Y, R Zhao, ME Belov, TP Conrads, GA Anderson, K Tang, L Pasa-Tolic, TD Veenstra, MS Lipton, HR Udseth, and RD Smith. 2001. "Packed Capillary Reversed-Phase Liquid Chromatography with High Performance Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Proteomics ." Analytical Chemistry 73(8):1766-1775. 
  • Bliss M, SM Bowyer, JL Bryant, MS Lipton, and KL Wahl. 2001. "Experiences and Management of Pregnant Radiation Workers at the Pacific Northwest National Laboratory." Chemical Safety and Health 8(3):6-8. 
  • Conrads TP, K Alving, TD Veenstra, ME Belov, GA Anderson, DJ Anderson, MS Lipton, L Pasa-Tolic, HR Udseth, WB Chrisler, BD Thrall, and RD Smith. 2001. "Quantitative Analysis of Bacterial and Mammalian Proteomes using a Combination of Cysteine Affinity Tags and 15N-Metabolic Labeling." Analytical Chemistry 73(9):2132-2139. 
  • Li L, CD Masselon, GA Anderson, L Pasa-Tolic, SW Lee, Y Shen, R Zhao, MS Lipton, TP Conrads, N Tolic, and RD Smith. 2001. "High-Throughput Peptide Identification from Protein Digests Using Data-Dependent Multiplexed Tandem FTICR Mass Spectrometry Coupled with Capillary Liquid Chromatography." Analytical Chemistry 73(14):3312-3322. 
  • Smith RD, L Pasa-Tolic, MS Lipton, PK Jensen, GA Anderson, Y Shen, TP Conrads, HR Udseth, R Harkewicz, ME Belov, CD Masselon, and TD Veenstra. 2001. "Rapid quantitative measurements of proteomes by Fourier transform ion cyclotron resonance mass spectrometry." Electrophoresis 22:1652-1668. 


  • Jensen PK, L Pasa-Tolic, KK Peden, S Martinovic, MS Lipton, GA Anderson, N Tolic, KK Wong, and RD Smith. 2000. "Mass Spectrometric Detection for Capillary Isoelectric Focusing Separations of Complex Protein Mixtures." Electrophoresis 21(7):1372-1380. 
  • Xiang F, GA Anderson, TD Veenstra, MS Lipton, and RD Smith. 2000. "Characterization of Microorganisms and Biomarker Development from Global ESI-MS/MS Analyses of Cell Lysates." Analytical Chemistry 72(11):2475-2481. 


  • Jensen PK, L Pasa-Tolic, GA Anderson, JA Horner, MS Lipton, JE Bruce, and RD Smith. 1999. "Probing Proteomes Using Capillary Isoelectric Focusing-Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry." Analytical Chemistry 71(11):2076-2084. 
  • Pasa-Tolic L, PK Jensen, GA Anderson, MS Lipton, KK Peden, S Martinovic, N Tolic, JE Bruce, and RD Smith. 1999. "High Throughput Proteome-Wide Precision Measurements of Protein Expression using Mass Spectrometry." Journal of the American Chemical Society 121(34):7949-7950. 

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