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Fundamental and Computational Sciences Directorate

Staff information

William Chrisler

Chem Bio & Exposure Sci Team
Biomedical Scientist
Pacific Northwest National Laboratory
PO Box 999
MSIN: J4-02
Richland, WA 99352

PNNL Publications


  • Cui Y., D. Hu, L. Markillie, W.B. Chrisler, M.J. Gaffrey, C.K. Ansong, and L. Sussel, et al. 2018. "Fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) for accurate detection and counting of RNA copies in single cells." Nucleic Acids Research 46, no. 2:Article No. e7. PNNL-SA-126413. doi:10.1093/nar/gkx874
  • Khan N.E., Y. Maezato, R.S. McClure, C.J. Brislawn, J.M. Mobberley, N.G. Isern, and W.B. Chrisler, et al. 2018. "Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture." Scientific Reports 8. PNNL-SA-129337. doi:10.1038/s41598-017-18630-1


  • Bernstein H.C., R.S. McClure, V. Thiel, N.C. Sadler, Y. Kim, W.B. Chrisler, and E.A. Hill, et al. 2017. "Indirect interspecies regulation; transcriptional and physiological responses of a cyanobacterium to heterotrophic partnership." mSystems 2, no. 2:Article No. e00181-16. PNNL-SA-113682. doi:10.1128/mSystems.00181-16
  • Boenisch M.J., K.L. Broz, S.O. Purvine, W.B. Chrisler, C.D. Nicora, L. Connolly, and M. Freitag, et al. 2017. "Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis." Scientific Reports 7. PNNL-SA-125650. doi:10.1038/srep44296
  • Hill E.A., W.B. Chrisler, A.S. Beliaev, and H.C. Bernstein. 2017. "A flexible microbial co-culture platform for simultaneous utilization of methane and carbon dioxide from gas feedstocks." Bioresource Technology 228. PNNL-SA-122746. doi:10.1016/j.biortech.2016.12.111
  • Kowalczyk J.E., C. Khosravi, S.O. Purvine, A. Dohnalkova, W.B. Chrisler, G. Orr, and E.W. Robinson, et al. 2017. "High resolution visualization and exo-proteomics reveal the physiological role of XlnR and AraR in plant biomass colonization and degradation by Aspergillus niger." Environmental Microbiology 19, no. 11:4587-4598. PNNL-SA-134692. doi:10.1111/1462-2920.13923
  • Liberton M.L., W.B. Chrisler, C.D. Nicora, R.J. Moore, R.D. Smith, D.W. Koppenaal, and H.B. Pakrasi, et al. 2017. "Phycobilisome Truncation Causes Widespread Proteome Changes in Synechocystis sp. PCC 6803." PLoS One 12, no. 3:e0173251. PNNL-SA-112677. doi:10.1371/journal.pone.0173251
  • Lindemann S.R., J.M. Mobberley, J.K. Cole, L. Markillie, R.C. Taylor, E.L. Huang, and W.B. Chrisler, et al. 2017. "Predicting Species-Resolved Macronutrient Acquisition during Succession in a Model Phototrophic Biofilm Using an Integrated ‘Omics Approach." Frontiers in Microbiology 8. PNNL-SA-129376. doi:10.3389/fmicb.2017.01020
  • Renslow R.S., M.J. Marshall, A.E. Tucker, W.B. Chrisler, and X. Yu. 2017. "In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel." Analyst 142, no. 13:2363-2371. PNNL-SA-109594. doi:10.1039/C7AN00078B


  • Bernstein H.C., R.S. McClure, E.A. Hill, L.M. Markillie, W.B. Chrisler, M.F. Romine, and J.E. McDermott, et al. 2016. "Unlocking the Constraints of Cyanobacterial Productivity: Acclimations Enabling Ultrafast Growth." mBio 7, no. 4:Article No. e00949-16. PNNL-SA-112394. doi:10.1128/mBio.00949-16
  • Mitchell H.D., L. Markillie, W.B. Chrisler, M.J. Gaffrey, D. Hu, C.J. Szymanski, and Y. Xie, et al. 2016. "Cells Respond to Distinct Nanoparticle Properties with Multiple Strategies as Revealed by Single-Cell RNA-Seq." ACS Nano 10, no. 11:10173-10185. PNNL-SA-121323. doi:10.1021/acsnano.6b05452
  • Shi T., M. Niepel, J.E. McDermott, Y. Gao, C.D. Nicora, W.B. Chrisler, and L.M. Markillie, et al. 2016. "Conservation of Protein Abundance Patterns Reveals the Regulatory Architecture of the of the EGFR-MAPK Pathway." Science Signaling 9, no. 436:rs6. PNNL-SA-115056. doi:10.1126/scisignal.aaf0891
  • Stegen J.C., J.K. Fredrickson, M.J. Wilkins, A. Konopka, W.C. Nelson, E.V. Arntzen, and W.B. Chrisler, et al. 2016. "Groundwater-Surface Water Mixing Shifts Ecological Assembly Processes and Stimulates Organic Carbon Turnover." Nature Communications 7. PNNL-SA-111962. doi:10.1038/ncomms11237


  • Jacobson K.H., I.L. Gunsolus, T.R. Kuech, J.M. Troiano, E.S. Melby, S.E. Lohse, and D. Hu, et al. 2015. "Lipopolysaccharide density and structure govern the extent and distance of nanoparticle interaction with actual and model bacterial outer membranes." Environmental Science & Technology 49, no. 17:10642-10652. PNNL-SA-112154. doi:10.1021/acs.est.5b01841
  • Mihai C., W.B. Chrisler, Y. Xie, D. Hu, C.J. Szymanski, A. Tolic, and J. Klein, et al. 2015. "Intracellular accumulation dynamics and fate of zinc ions in alveolar epithelial cells exposed to airborne ZnO nanoparticles at the air-liquid interface." Nanotoxicology 9, no. 1:9-22. PNNL-SA-99125. doi:10.3109/17435390.2013.859319
  • von Neubeck C., M. Geniza, P.M. Kauer, J.E. Robinson, W.B. Chrisler, and M.B. Sowa. 2015. "The effect of low dose ionizing radiation on homeostasis and functional integrity in an organotypic human skin model." Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis 775. PNNL-SA-106741. doi:10.1016/j.mrfmmm.2015.03.003


  • Beliaev A.S., M.F. Romine, M. Serres, H.C. Bernstein, B.E. Linggi, L.M. Markillie, and N.G. Isern, et al. 2014. "Inference of Interactions in Cyanobacterial-Heterotrophic Co-Cultures via Transcriptome Sequencing." The ISME Journal 8, no. 11:2243-2255. PNNL-SA-98626. doi:10.1038/ismej.2014.69
  • Cole J.K., J.R. Hutchison, R.S. Renslow, Y. Kim, W.B. Chrisler, H.E. Engelmann, and A. Dohnalkova, et al. 2014. "Phototrophic Biofilm Assembly in Microbial-Mat-Derived Unicyanobacterial Consortia: Model Systems for the Study of Autotroph-Heterotroph Interactions." Frontiers in Microbiology 5. PNNL-SA-99657. doi:10.3389/fmicb.2014.00109
  • Hua X., X. Yu, Z. Wang, L. Yang, B. Liu, Z. Zhu, and A.E. Tucker, et al. 2014. "In situ molecular imaging of hydrated biofilm in a microfluidic reactor by ToF-SIMS." Analyst 139. PNNL-SA-99617. doi:10.1039/C3AN02262E
  • Sadler N.C., M.R. Melnicki, M.H. Serres, E.D. Merkley, W.B. Chrisler, E.A. Hill, and M.F. Romine, et al. 2014. "Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium." ACS Chemical Biology 9, no. 1:291-300. PNNL-SA-93812. doi:10.1021/cb400769v
  • Shi T., Y. Gao, M.J. Gaffrey, C.D. Nicora, T.L. Fillmore, W.B. Chrisler, and M.A. Gritsenko, et al. 2014. "Sensitive Targeted Quantification of ERK Phosphorylation Dynamics and Stoichiometry in Human Cells without Affinity Enrichment." Analytical Chemistry 87, no. 2:1103-1110. PNNL-SA-103421. doi:10.1021/ac503797x
  • Wiedner S.D., L.N. Anderson, N.C. Sadler, W.B. Chrisler, V.K. Kodali, R.D. Smith, and A.T. Wright. 2014. "Organelle-Specific Activity-Based Protein Profiling in Living Cells." Angewandte Chemie International Edition 53, no. 11:2919-2922. PNNL-SA-98713. doi:10.1002/anie.201309135


  • von Neubeck C., H. Shankaran, M. Geniza, P.M. Kauer, R.J. Robinson, W.B. Chrisler, and M.B. Sowa. 2013. "Integrated Experimental and Computational Approach to Understand the Effects of Heavy Ion Radiation on Skin Homeostasis." Integrative Biology 5, no. 10:1229-1243. PNNL-SA-94876. doi:10.1039/C3IB40071A
  • Welkie D.G., D.M. Sherman, W.B. Chrisler, G. Orr, and L.A. Sherman. 2013. "Analysis of carbohydrate storage granules in the diazotrophic cyanobacterium Cyanothece sp. PCC 7822." Photosynthesis Research 118, no. 1-2:25-36. PNNL-SA-98888. doi:10.1007/s11120-013-9941-z


  • Freiin von Neubeck C.H., H. Shankaran, N.J. Karin, P.M. Kauer, W.B. Chrisler, X. Wang, and R.J. Robinson, et al. 2012. "Cell Type-dependent Gene Transcription Profile in Three Dimensional Human Skin Tissue Model Exposed to Low Doses of Ionizing Radiation: Implications for Medical Exposures." Environmental and Molecular Mutagenesis 53, no. 4:247-259. PNNL-SA-79289. doi:10.1002/em.21682
  • Jin H., Y. Yu, W.B. Chrisler, Y. Xiong, D. Hu, and C. Lei. 2012. "Delivery of MicroR A-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing." Breast Cancer: Basic and Clinical Research 6. PNWD-SA-9642. doi:10.4137/BCBCR.S8513
  • Shankaran H., Y. Zhang, W.B. Chrisler, J.A. Ewald, H.S. Wiley, and H. Resat. 2012. "Integrated Experimental and Model-based Analysis Reveals the Spatial Aspects of EGFR Activation Dynamics." Molecular Biosystems 8, no. 11:2868-2882. PNNL-SA-80743. doi:10.1039/C2MB25190F
  • Waters K.M., D.L. Stenoien, M.B. Sowa, C.H. Freiin Von Neubeck, W.B. Chrisler, R. Tan, and R.L. Sontag, et al. 2012. "Annexin A2 Modulates Radiation-Sensitive Transcriptional Programming and Cell Fate." Radiation Research 179, no. 1:56-61. PNNL-SA-84443. doi:10.1667/RR3056.1
  • Xie Y., N.G. Williams, A. Tolic, W.B. Chrisler, J.G. Teeguarden, B.L. Maddux, and J.G. Pounds, et al. 2012. "Aerosolized ZnO nanoparticles induce toxicity in alveolar type II epithelial cells at the air-liquid interface." Toxicological Sciences 125, no. 2:450-461. PNNL-SA-83610. doi:10.1093/toxsci/kfr251


  • Miller J.H., A. Suleiman, W.B. Chrisler, and M.B. Sowa. 2011. "Simulation of Electron-Beam Irradiation of Skin Tissue Model." Radiation Research 175, no. 1:113-118. PNNL-SA-73413. doi:10.1667/RR2339.1
  • Miller J.H., W.B. Chrisler, X. Wang, and M.B. Sowa. 2011. "Confocal Microscopy for Modeling Electron Microbeam Irradiation of Skin." Radiation and Environmental Biophysics 50, no. 3:365-369. PNNL-SA-80079. doi:10.1007/s00411-011-0371-z
  • Orr G., W.B. Chrisler, K.J. Cassens, R. Tan, B.J. Tarasevich, L.M. Markillie, and R.C. Zangar, et al. 2011. "Cellular Recognition and Trafficking of Amorphous Silica Nanoparticles by Macrophage Scavenger Receptor A." Nanotoxicology 5, no. 3:296-311. PNNL-SA-68643. doi:10.3109/17435390.2010.513836


  • Hinderliter P.M., K.R. Minard, G. Orr, W.B. Chrisler, B.D. Thrall, J.G. Pounds, and J.G. Teeguarden. 2010. "ISDD: A Computational Model of Particle Sedimentation, Diffusion and Target Cell Dosimetry for In Vitro Toxicity Studies." Particle and Fibre Toxicology 7, no. November:Article No. 36. PNNL-SA-74873. doi:10.1186/1743-8977-7-36
  • Shankaran H., W.B. Chrisler, R.L. Sontag, and T.J. Weber. 2010. "Inhibition of ERK Oscillations by Ionizing Radiation and Reactive Oxygen Species." Molecular Carcinogenesis 50, no. 6:424-432. PNNL-SA-74660. doi:10.1002/mc.20724
  • Sowa M.B., W.B. Chrisler, K.D. Zens, E.J. Ashjian, and L.K. Opresko. 2010. "Three-dimensional Culture Conditions Lead to Decreased Radiation Induced Crytoxicity in Human Mammary Epithelial Cells." Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis 687, no. 1-2:78-83. PNNL-SA-71310. doi:10.1016/j.mrfmmm.2010.03.004
  • Weber T.J., H. Shankaran, H.S. Wiley, L.K. Opresko, W.B. Chrisler, and R.D. Quesenberry. 2010. "Basic Fibroblast Growth Factor Regulates Persistent ERK Osciliations in Premaligant but not Malignant JB6 Cells." Journal of Investigative Dermatology 130, no. 5:144-156. PNNL-SA-59833.


  • Karagiosis S.A., W.B. Chrisler, N. Bollinger, and N.J. Karin. 2009. "Lysophosphatidic Acid-induced ERK Activation and Chemotaxis in MC3T3-E1 Preosteoblasts are Independent of EGF Receptor Transactivation." Journal of Cellular Physiology 219, no. 3:716-723. PNNL-SA-62926.
  • Shankaran H., D.L. Ippolito, W.B. Chrisler, H. Resat, N. Bollinger, L.K. Opresko, and H.S. Wiley. 2009. "Rapid and Sustained Nuclear-Cytoplasmic ERK Oscillations Induced by Epidermal Growth Factor." Molecular Systems Biology 5. PNNL-SA-54421. doi:10.1038/msb.2009.90
  • Waters K.M., R. Tan, L.K. Opresko, R.D. Quesenberry, S. Bandyopadhyay, W.B. Chrisler, and T.J. Weber. 2009. "Cellular Dichotomy Between Anchorage-Independent Growth Responses to bFGF and TA Reflects Molecular Switch in Commitment to Carcinogenesis." Molecular Carcinogenesis 48, no. 11:1059-1069. PNNL-SA-57090.
  • Zhang Y., L.K. Opresko, H. Shankaran, W.B. Chrisler, H.S. Wiley, and H. Resat. 2009. "HER/ErbB Receptor Interactions and Signaling Patterns in Human Mammary Epithelial Cells." BMC Cell Biology 10. PNNL-SA-64538. doi:10.1186/1471-2121-10-78


  • Coleman J.R., D.E. Culley, W.B. Chrisler, and F.J. Brockman. 2007. "mRNA-targeted Fluorescent in Situ Hybridization (FISH) of Gram-negative Bacteria Without Template Amplification or Tyramide Signal Amplification." Journal of Microbiological Methods 71, no. 3:246-255. PNNL-SA-55589. doi:10.1016/j.mimet.2007.09.011


  • Dong J., L. Opresko, W.B. Chrisler, G. Orr, R.D. Quesenberry, D.A. Lauffenburger, and H.S. Wiley. 2005. "The Membrane-anchoring Domain of Epidermal Growth Factor Receptor Ligands Dictates Their Ability to Operate in Juxtacrine Mode." Molecular Biology of the Cell 16, no. 6:2984-2998. PNWD-SA-7019.
  • Weber T.J., R.W. Siegel, L. Markillie, W.B. Chrisler, X.C. Lei, and N.H. Colburn. 2005. "A paracrine signal mediates the cell transformation response to low dose gamma radiation in JB6 cells." Molecular Carcinogenesis 43, no. 1:31-37. PNNL-SA-41438.


  • Jasionowski M., K.J. Krzyminski, W.B. Chrisler, L.M. Markillie, J.E. Morris, and A. Gutowska. 2004. "Thermally-reversible gels for 3D cell culture of chondrocytes." Journal of Materials Science. Materials in Medicine 15, no. 5:575-582. PNNL-SA-43425.


  • Weber T.J., L.M. Markillie, W.B. Chrisler, G.A. Vielhauer, and J.W. Regan. 2002. "Modulation of JB6 Mouse Epidermal Cell Transformation Response by the Prostaglandin F2 alpha Receptor." Molecular Carcinogenesis 35, no. 4:163-172. PNNL-SA-38182.
  • Weber T.J., L.M. Markillie, W.B. Chrisler, G.A. Vielhauer, and J.W. Regan. 2002. "Modulation of JB6 Mouse Epidermal Cell Transformation Response by the Prostaglandin F2 Alpha Receptor." Molecular Carcinogenesis 35, no. 4:163-172. PNWD-SA-5669.


  • Conrads T.P., K. Alving, T.D. Veenstra, M.E. Belov, G.A. Anderson, D.J. Anderson, and M.S. Lipton, et al. 2001. "Quantitative Analysis of Bacterial and Mammalian Proteomes using a Combination of Cysteine Affinity Tags and 15N-Metabolic Labeling." Analytical Chemistry 73, no. 9:2132-2139. PNNL-SA-34135.
  • Gutowska A., M. Jasionowski, J.E. Morris, W.B. Chrisler, Y.H. An, and V. Mironov. 2001. "Polymer Formulations for Cartilage Repair." In Transactions: 27th annual meeting, Society for Biomaterials, in conjunction with the 33rd International Biomaterials Symposium, edited by LT Khun and CM Agrawal, 24, 566. Minneapolis, Minnesota:Society for Biomaterials. PNNL-SA-34785.

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