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Center for Genomic Experimentation and Computation

Roger Brent, Principal Investigator, Molecular Sciences Institute
David Camp, PNNL Contact

Funding Agency: National Institutes of Health, National Human Genome Research Institute

The Center for Genomic Experimentation and Computation has been established by the project team at the Molecular Sciences Institute and Pacific Northwest National Laboratory (PNNL). The Center's purpose is to combine functional genomic and computational research to model a prototype signal transduction pathway. Work at the Center is focused on the Alpha Project, the overall goal of which is to predict the behavior of the G-protein receptor coupled signal transduction pathway, which governs the response of haploid MATa S. cerevisiae to the yeast mating pheromone. This pathway is a prototype for regulatory networks that govern response to external stimuli in higher eukaryotes. It is sufficiently tractable to facilitate development of the numerous functional genomic experimental and computational methods and sufficiently paradigmatic so that successful experimental and computational tactics can be ported rapidly to other systems in other organisms.

The role of researchers at Pacific Northwest National Laboratory (PNNL) is to develop mass spectrometry- (MS-) based methods to analyze and quantify phosphorylated and unphosphorylated species of signal transduction proteins in the yeast pheromone pathway. The overall goals of the Center project team are to (1) develop experimental means to measure system output and key intermediate quantities from single cells and populations of cells, (2) develop computational means to simulate the behavior of cells and populations of cells, (3) use these methods to build models that predict the quantitative behavior of cells over time and in response to defined perturbations, and (4) accomplish heuristic goals including learning to perform combined experimental work and learning to develop investigators in a multidisciplinary genomic research environment.

Work at the Center will deepen our understanding of important but poorly understood scientific questions, including the extent and importance of epigenetic variation and the means by which dynamic and quantitative aspects of biological system behavior are controlled. Researchers working on the Center project will develop functional genomic and computational methods that are scalable to systematic large-scale data collection and that are applicable to similar studies of other organisms, including humans.

Information for this webpage was taken from the NIH Center for Genomic Experimentation and Computation project page.

Systems Biology at PNNL

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