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H. Steven Wiley, Ph. D.

Director, Biomolecular Systems

Dr. H. Steven Wiley
Dr. H. Steven Wiley, Director


Dr. Wiley was a pioneer in developing methods for the quantitative analysis of receptor dynamics in mammalian cells and published some of the first computer models of receptor regulation. He has been a major contributor to the field of receptor research, particularly with regard to the control of receptor distribution within cells. He joined Pacific Northwest National Laboratory in 2000 to develop a systems biology program to exploit the laboratory's unique capabilities in cell imaging, computational biology and high-throughput proteomics. Currently, he is Lead Biologist at EMSL, a U.S. Department of Energy national scientific user facility located at PNNL. His research interests are focused on understanding mechanisms of cell regulatory networks using both prokaryotic and eukaryotic model systems. This work also involves mapping cellular signal transduction networks and how these networks integrate information from multiple inputs. More recently, his work has focused on building predictive models of the EGFR autocrine circuit using a combination of proteomics, gene expression, biochemical and optical assays. This work requires the creation of a scalable computational infrastructure for integrating multidimensional data sets as well as development of the underlying analytical technologies.

Dr. Wiley is also leading the development of a Systems Microbiology and Extremophile Research Facility in the EMSL that will provide the scientific community access to the technologies needed for systems biology research on microbial systems.


B.A. in Biology from the University of Tennessee; Ph.D. in Biomedical Sciences from the University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences. Postdoctoral fellow with Dennis Cunningham at University of California, Irvine.


Predoctoral and Postdoctorial fellowships from the National Institutes of Health; Research Career Development Award from the National Institutes of Health; awarded research grants from the NIH, the National Science Foundation, the American Cancer Society, the Department of Defense and the Department of Energy; served as a reviewer for NASA, the NIH, the NCI, the NSF and multiple international granting agencies. He has been invited speaker at numerous national and international scientific conferences and is on the advisory panel for the Burroughs Welcome Fund. Received an R&D 100 award for designing a single-chain antibody library in a yeast-display system. Elected Fellow of the American Association for the Advancement of Science.


Author or co-author of more than 160 scientific publications, including more than 100 articles in peer-reviewed scientific journals and 20 review articles and book chapters. He also has written commercial graphics and data analysis software packages. Dr. Wiley has served as a reviewer for more than 30 scientific journals and writes a monthly column for The Scientist.

Shankaran H, H Resat, and HS Wiley. 2007. “Cell surface receptors for signal transduction and ligand transport - a design principles study.” PLoS Comput. Biol. 3: e101.

Rodland KD, N Bollinger, D Ippolito, LK Opresko, RJ Coffey, R Zangar, and HS Wiley. 2008. “Multiple Mechanisms are Responsible for Transactivation of the Epidermal Growth Factor Receptor in Mammary Epithelial Cells.” J. Biol. Chem. 283: 31477-31487.

Rogers S, M Girolami, W Kolch, KM Waters, T Liu, B Thrall, and HS Wiley. 2008. “Investigating the correspondence between transcriptomic and proteomic expression profiles using coupled cluster models.” Bioinformatics 24: 2894-2900.

Shankaran H, and HS Wiley. 2008. “Smad signaling dynamics: insights from a parsimonious model.” Science signaling 1: pe41.

Contact Information

Systems Biology at PNNL

Research & Capabilities