Nuclear Magnetic Resonance & Electron Paramagnetic Resonance
The William R. Wiley Environmental Molecular Sciences Laboratory's 900-MHz nuclear magnetic resonance spectrometer being tuned and calibrated for a triple-resonance experiment of a 66 kDa protein-lipid complex.
Nuclear Magnetic Resonance (NMR) and Electron Paramagnetic Resonance (EPR) instruments at Pacific Northwest National Laboratory (PNNL) are housed in the High-Field Magnetic Resonance Facility (HFMRF), which is part of the Environmental Molecular Sciences Laboratory (EMSL). Current research in the HFMRF is focused on determining the structures of molecules that could affect biological health and remediation of environmental damage.
Our scientists have used the facility to carry out some of the most exciting research in modern molecular biology and biochemistry, including:
- structural genomics - to determine the three-dimensional structure of DNA, RNA, proteins, and enzymes as well as their intermolecular association
- functional genomics - to determine the interaction domains among DNA, RNA, and proteins, with particular emphasis on DNA damage recognition and repair processes
- biological imaging - to acquire images and corresponding chemical information in biological samples, with particular interest in development of combined magnetic resonance and optical spectroscopy techniques to elucidate biological processes
- solid state - for low gamma nuclei detection and slow magic-angle spinning methodologies for cells, tissues, small animals, and bacterial colonies
- novel low-temperature solid-state NMR spectroscopy - for sensitivity enhancement; this technique has been applied to allow direct observation of the Zn2+ in carbonic anhydrase (highlight).
Instrumentation and TechnologiesNote: The following links are to the EMSL website
- 900-MHz, NMR
- 750-MHz NMR
- 600-MHz Varian Inova NMR
- 500-MHz Bruker Avance WB NMR
- 500-MHz CMX NB NMR
- 500-MHz Varian Unity NB NMR
- 400-MHz WB NMR
- 300 CMX MHz WB NMR
- EPR Spectrometer with ENDOR (Electron-Nuclear Double Resonance)/ELDOR (Electron Double Resonance) capability
- Combined optical and magnetic resonance microscope
- Low temperature probes for metalloprotein chemistry and structure
- Virtual NMR capability enables use and collaboration with EMSL scientists for remote users via secure shell over the Internet