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Staff information


Kim Hixson

Environmental Dynamics & Simulations
Pacific Northwest National Laboratory
PO Box 999
MSIN: J4-02
Richland, WA 99352


As part of the EMSL mission, Hixson has been the science point of contact for multiple EMSL user projects, overseeing and facilitating the progression of successful data accumulation and interpretation. Her major projects now include full multi-omic comparisons and data interpretations for challenging plant-focused omics projects, including soybean symbiotic relationships in collaboration with scientists from the University of Missouri and characterization of the phenylpropanoid pathway in Arabidopsis thaliana and characterization of phenylethanol producing Poplar trees (engineered to produced high value chemicals and high energy biofuels) in collaboration with Dr. Norman Lewis from Washington State University. The soybean study will enhance knowledge of symbiotic relationships between plants and nitrogenase-containing bacteria that may eventually aid in the development of plants that thrive without the need for fertilizers. The Arabidopsis and Poplar tree projects will aid in understanding of how plants can be efficiently engineered to produce many highly valuable chemicals. Many of these chemicals could serve as a renewable resource for chemicals and fuels currently obtained from petroleum. Current research also includes the genome sequencing of the nitrogen-fixing tree red alder, along with molecular characterization of the tree in association with microbial symbionts.

Research Interests

  • Advanced Proteomics strategies/methodologies
  • Multiomics integration(proteomics, transcriptomics, metabolomics, etc)
  • Plant and photosynthetic organism related research.

Education and Credentials

  • Ph.D. Molecular Plant Sciences from Washington State University (2016)
  • M.S. Chemistry from Washington State University (2005)
  • B.S. Chemistry from Eastern Oregon University (1997)

Awards and Recognitions

  • 2014: ACS Withycombe-Charalambous Symposium Award
  • 2011-2012: Helen and Loyal H. Davis Fellowship Award
  • 2011: 61st Lindau Nobel Laureate Meeting Selected Young Scientist Participant
  • 2011: Phytochemical Society of North America Best Poster Award
  • 2010-2011: NIH Biotechnology Training Program
  • 2006-2008: Battelle Pacific Northwest National Laboratory Scientist and Engineer Development Program
  • 2004: Fundamental Sciences Directorate Outstanding Performance Award for Contribution to the Proteomic Characterization of the Photosystem II Protein Complex in Cyanobacterium synechocystis
  • 2001: Environmental Health Sciences Division Outstanding Performance Award for the Pilot Proteome Project of the Pacific Northwest National Laboratory
  • 1997: US DOE Associated Western Universities Fellowship at PNNL.

PNNL Publications


  • Handakumbura P, KK Hixson, SO Purvine, GC Jansson, and L Pasa Tolic. 2017. "Plant iTRAQ-based proteomics." Current Protocols in Plant Biology 2(2):158-172.  doi:10.1002/cppb.20052
  • Strand DD, AK Livingston, M Satoh-Cruz, T Koepke, HM Enlow, N Fisher, JE Froehlich, JA Cruz, D Minhas, KK Hixson, K Kohzuma, MS Lipton, A Dhingra, and D Kramer. 2017. "Defects in the Expression of Chloroplast Proteins Leads to H2O2 Accumulation and Activation of Cyclic Electron Flow around Photosystem I." Frontiers in Plant Science 7:2073.  doi:10.3389/fpls.2016.02073
  • White RA, III, A Rivas-Ubach, MI Borkum, M Koberl, A Bilbao Pena, SM Colby, DW Hoyt, AK Bingol, YM Kim, JP Wendler, KK Hixson, and GC Jansson. 2017. "The state of rhizospheric science in the era of multi-omics: A practical guide to omics technologies." Rhizosphere 3(2):212-221.  doi:10.1016/j.rhisph.2017.05.003


  • Valdes-Lopez O, JM Batek Rios, N Gomez-Hernandez, CT Nguyen, MC Isidra-Arellano, N Zhang, T Joshi, D Xu, KK Hixson, KK Weitz, JT Aldrich, L Pasa-Tolic, and G Stacey. 2016. "Soybean Roots Grown under Heat Stress Show Global Changes in Their Transcriptional and Proteomic Profiles." Frontiers in Plant Science 7:Article No. 517.  doi:10.3389/fpls.2016.00517


  • Nakayasu ES, S Wu, MA Sydor, AK Shukla, KK Weitz, RJ Moore, KK Hixson, JS Kim, VA Petyuk, ME Monroe, L Pasa-Tolic, W Qian, RD Smith, JN Adkins, and C Ansong. 2014. "A Method to determine lysine acetylation stoichiometries." International Journal of Proteomics 2014:Article No. 730725.  doi:10.1155/2014/730725


  • Nakayasu ES, RN Brown, C Ansong, MA Sydor, S Imtiaz, C Mihai, RL Sontag, KK Hixson, ME Monroe, T Sobreira, G Orr, VA Petyuk, F Yang, RD Smith, and JN Adkins. 2013. "Multi-omic data integration links Deleted in Breast Cancer 1 (DBC1) Degradation to Chromatin Remodeling in Inflammatory Response." Molecular & Cellular Proteomics. MCP 12(8):2136-2147.  doi:10.1074/mcp.M112.026138


  • Brechenmacher L, TH Nguyen, KK Hixson, M Libault, JT Aldrich, L Pasa-Tolic, and G Stacey. 2012. "Identification of soybean proteins from a single cell type: The root hair." Proteomics 12(22):3365-3373.  doi:10.1002/pmic.201200160
  • Nguyen TH, L Brechenmacher, JT Aldrich, TRW Clauss, MA Gritsenko, KK Hixson, M Libault, K Tanaka, F Yang, Q Yao, L Pasa-Tolic, D Xu, HT Nguyen, and G Stacey. 2012. "Quantitative Phosphoproteomic Analysis of Soybean Root Hairs Inoculated with Bradyrhizobium japonicum." Molecular & Cellular Proteomics. MCP 11(11):1140-1155.  doi:10.1074/mcp.M112.018028


  • Lopez-Ferrer D, K Petritis, EW Robinson, KK Hixson, Z Tian, JH Lee, SW Lee, N Tolic, KK Weitz, ME Belov, RD Smith, and L Pasa-Tolic. 2011. "Pressurized Pepsin Digestion in Proteomics: An Automatable Alternative to Trypsin for Integrated Top-down Bottom-up Proteomics." Molecular & Cellular Proteomics. MCP 10(2):, doi:10.1074/mcp.M110.001479
  • Lopez-Ferrer D, KK Hixson, ME Belov, and RD Smith. 2011. "Ultra-Fast Sample Preparation for High-Throughput Proteomics." Chapter 8 in Sample Preparation in Biological Mass Spectrometry, ed. AR Ivanov and AV Lazarev, pp. 125-139.  Springer, Dordrecht, Germany. 


  • Bagwell CE, KK Hixson, CE Milliken, D Lopez-Ferrer, and KK Weitz. 2010. "Proteomic and Physiological Responses of Kineococcus radiotolerans to Copper." PLoS One 5(8):Article No. e12427.  doi:10.1371/journal.pone.0012427
  • DiDonato RJ, ND Young, J Butler, KJ Chin, KK Hixson, P Mouser, MS Lipton, R DeBoy, and B Methe. 2010. "Genome Sequence of the Deltaproteobacterial Strain NaphS2 and Analysis of Differential Gene Expression during Anaerobic Growth on Naphthalene." PLoS One 5(11):Article No. e14072.  doi:10.1371/journal.pone.0014072
  • Gupta N, KK Hixson, DE Culley, RD Smith, and PA Pevzner. 2010. "Analyzing Protease Specificity and Detecting in Vivo Proteolytic Events Using Tandem Mass Spectrometry." Proteomics 10(15):2833-2844. 
  • Hixson KK, D Lopez-Ferrer, EW Robinson, and L Pasa-Tolic. 2010. "Proteomics." In Encyclopedia of Spectroscopy and Spectrometry, 2nd edition, ed. J Lindon, GE Trantor, D Koppenaal, pp. 2280-2288.  Academic Press, Amsterdam, Netherlands Antilles. 
  • Lewis NE, KK Hixson, TM Conrad, JA Lerman, P Charusanti, AD Polpitiya, JN Adkins, G Schramm, SO Purvine, D Lopez-Ferrer, KK Weitz, R Eils, R Konig, RD Smith, and BO Palsson. 2010. "Omic data from evolved E. coli are consistent with computed optimal growth from genome-scale models." Molecular Systems Biology 6:Article No. 390.  doi:10.1038/msb.2010.47
  • Schutzer SE, T Liu, B Natelson, TE Angel, AA Schepmoes, SO Purvine, KK Hixson, MS Lipton, DG Camp, II, PK Coyle, RD Smith, and J Bergquist. 2010. "Establishing the Proteome of Normal Human Cerebrospinal Fluid ." PLoS One 5(6):e10980-.  doi:10.1371/journal.pone.0010980


  • Kim W, MW Silby, SO Purvine, JS Nicoll, KK Hixson, ME Monroe, CD Nicora, MS Lipton, and SB Levy. 2009. "Proteomic Detection of Non-Annotated Protein-Coding Genes in Pseudomonas fluorescens Pf0-1." PLoS One 4(12):Article Number: e8455.  doi:10.1371/journal.pone.0008455
  • Lopez-Ferrer D, KK Hixson, HS Smallwood, TC Squier, K Petritis, and RD Smith. 2009. "Evaluation of a High Intensity Focused Ultrasound-Immobilized Trypsin Digestion and 18 O-Labeling Method for Quantitative Proteomics." Analytical Chemistry 81(15):6272-6277. 


  • Shen Y, KK Hixson, N Tolic, DG Camp, II, SO Purvine, RJ Moore, and RD Smith. 2008. "Mass Spectrometry Analysis of Proteome-wide Proteolytic Post-translational Degradation of Proteins." Analytical Chemistry 80(15):5819-5828.  doi:10.1021/ac800077w
  • Shen Y, N Tolic, KK Hixson, SO Purvine, GA Anderson, and RD Smith. 2008. "De novo sequencing of unique sequence tags for discovery of post-translational modifications of proteins ." Analytical Chemistry 80(20):7742-7754.  doi:10.1021/ac801123p
  • Ding YHR, KK Hixson, M Aklujkar, MS Lipton, RD Smith, DR Lovley, and T Mester. 2008. "Proteome of Geobacter sulfurreducens grown with Fe(III) oxide or Fe(III) citrate as the electron acceptor." Biochimica et Biophysica Acta--Proteins and Proteomics 1784(12):1935-1941.  doi:10.1016/j.bbapap.2008.06.011
  • Lopez-Ferrer D, K Petritis, KK Hixson, TH Heibeck, RJ Moore, ME Belov, DG Camp, II, and RD Smith. 2008. "Application of pressurized solvents for ultrafast trypsin hydrolysis in proteomics: Proteomics on the fly." Journal of Proteome Research 7(8):3276-3281.  doi:10.1021/pr7008077
  • Lopez-Ferrer D, K Petritis, NM Lourette, BH Clowers, KK Hixson, TH Heibeck, DC Prior, L Pasa-Tolic, DG Camp, II, ME Belov, and RD Smith. 2008. "Fast On-Line Digestion System for Protein Characterization ." Analytical Chemistry 80(23):8930-8936.  doi:10.1021/ac800927v
  • Lopez-Ferrer D, TH Heibeck, K Petritis, KK Hixson, W Qian, ME Monroe, AM Mayampurath, RJ Moore, ME Belov, DG Camp, II, and RD Smith. 2008. "Rapid Sample Processing For LC-MS Based Quantitative Proteomics Using High Intensity Focused Ultrasounds." Journal of Proteome Research 7(9):3860-3867.  doi:10.1021/pr800161x
  • Shen Y, N Tolic, KK Hixson, SO Purvine, L Pasa-Tolic, W Qian, JN Adkins, RJ Moore, and RD Smith. 2008. "Proteome-wide identification of proteins and their modifications with decreased ambiguities and improved false discovery rates using unique sequence tags." Analytical Chemistry 80(6):1871-82.  doi:10.1021/ac702328x
  • Wegener KM, EA Welsh, LE Thornton, NS Keren, JM Jacobs, KK Hixson, ME Monroe, DG Camp, II, RD Smith, and HB Pakrasi. 2008. "High Sensitivity Proteomics Assisted Discovery of a Novel Operon Involved in the Assembly of Photosystem II, a Membrane Protein Complex." Journal of Biological Chemistry 283(41):27829-27837.  doi:10.1074/jbc.M803918200


  • Fried M, KK Hixson, L Anderson, Y Ogata, TK Mutabingwa, and PE Duffy. 2007. "The distinct proteome of placental malaria parasites." Molecular and Biochemical Parasitology 155(1):57-65.  doi:10.1016/j.molbiopara.2007.05.010
  • Resch W, KK Hixson, RJ Moore, MS Lipton, and B Moss. 2007. "Protein Composition of the Vaccinia Virus Mature Virion." Virology 358(1):233-247. 


  • Ding YHR, KK Hixson, CS Giometti, A Stanley, A Esteve-Nunez, T Khare, SL Tollaksen, W Zhu, JN Adkins, MS Lipton, RD Smith, T Mester, and DR Lovley. 2006. "The Proteome of Dissimilatory Metal-reducing Microorganism Geobacter Sulfurreducens under Various Growth Conditions." Biochimica et Biophysica Acta--Proteins and Proteomics 1764(7):1198-1206.  doi:10.1016/j.bbapap.2006.04.017
  • Hixson KK, JN Adkins, SE Baker, RJ Moore, RD Smith, SL McCutchen-Maloney, and MS Lipton. 2006. "Biomarker Candidate Identification in Yersinia Pestis Using Organism-Wide Semiquantitative Proteomics ." Journal of Proteome Research 5(11):3008-3017. 
  • Lipton MS, MF Romine, ME Monroe, DA Elias, L Pasa-Tolic, GA Anderson, DJ Anderson, JK Fredrickson, KK Hixson, CD Masselon, HM Mottaz, N Tolic, and RD Smith. 2006. "AMT Tag Approach to Proteomic Characterization of Deinococcus Radiodurans and Shewanella Oneidensis ." In Microbial Proteomics, Methods of Biochemical Analysis, vol. 49, ed. I. Humphery-Smith and M. Hecker, pp. 113-134.  John Wiley & Sons, Inc., Hoboken, NJ. 
  • Luo Q, K Tang, F Yang, A Elias, Y Shen, RJ Moore, R Zhao, KK Hixson, SS Rossie, and RD Smith. 2006. "More sensitive and quantitative proteomic measurements using very low flow rate porous silica monolithic LC columns with electrospray ionization-mass spectrometry ." Journal of Proteome Research 5(5):1091-1097. 


  • Shen Y, R Zhang, RJ Moore, J Kim, TO Metz, KK Hixson, R Zhao, EA Livesay, HR Udseth, and RD Smith. 2005. "Automated 20 kpsi RPLC-MS and MS/MS with chromatographic peak capacities of 1000-1500 and capabilities in proteomics and metabolomics ." Analytical Chemistry 77(10):3090-3100. 
  • Luo Q, Y Shen, KK Hixson, R Zhao, F Yang, RJ Moore, HM Mottaz, and RD Smith. 2005. "Preparation of 20-µm-i.d. Silica-based Monolithic Columns and Application for Proteomic Analysis." Analytical Chemistry 77(15):5028-5035. 


  • Shen Y, N Tolic, CD Masselon, L Pasa-Tolic, DG Camp, II, KK Hixson, R Zhao, GA Anderson, and RD Smith. 2004. "Ultrasensitive Proteomics using High-Efficiency on-Line Micro-SPE-NanoLC-NanoESI MS and MS/MS." Analytical Chemistry 76(1):144-154. 
  • Prokisch H, CM Scharfe, DG Camp, II, W Xiao, L David, C Andreoli, ME Monroe, RJ Moore, MA Gritsenko, C Kozany, KK Hixson, HM Mottaz, H Zischka, M Ueffing, ZS Herman, RW Davis, T Meitinger, P Oefner, RD Smith, and LM Steinmetz. 2004. "Integrative Analysis of the Mitochondrial Proteome in Yeast." PloS Biology 2(6):0795-0804. 


  • Shen Y, RJ Moore, R Zhao, J Blonder, DL Auberry, CD Masselon, L Pasa-Tolic, KK Hixson, KJ Auberry, and RD Smith. 2003. "High-Efficiency On-Line Solid-Phase Extraction Coupling to 15-150 um I.D. Column Liquid Chromatography for Proteomic Analysis." Analytical Chemistry 75(14):3596-3605. 
  • Mohan D, L Pasa-Tolic, CD Masselon, N Tolic, B Bogdanov, KK Hixson, RD Smith, and CS Lee. 2003. "Integration of Electrokinetic-Based Multidimensional Separations/Concentration Platform with electrospray ionization-Fourier transform ion cyclotron resonance-mass spectrometry for Proteome Analysis of Shewanella Oneidensis." Analytical Chemistry 75(17):4432-4440. 


  • Hixson KK, N Rodriguez, DG Camp, II, EF Strittmatter, MS Lipton, and RD Smith. 2002. "Evaluation of Enzymatic Digestion and Liquid Chromatography-Mass Spectrometry Peptide Mapping of the Integral Membrane Protein Bacteriorhodopsin." Electrophoresis 23(18):3224-3232. 
  • Lipton MS, L Pasa-Tolic, GA Anderson, DJ Anderson, DL Auberry, JR Battista, MJ Daly, JK Fredrickson, KK Hixson, HM Kostandarithes, CD Masselon, LM Markillie, RJ Moore, MF Romine, Y Shen, EF Strittmatter, N Tolic, HR Udseth, A Venkateswaran, KK Wong, R Zhao, and RD Smith. 2002. "Global Analysis of Deinococcus Radiodurans Proteome by Csing Accurate Mass Tags." Proceedings of the National Academy of Sciences of the United States of America 99(17):11049-11054. 


  • Pasa-Tolic L, PK Jensen, GA Anderson, MS Lipton, KK Peden, S Martinovic, N Tolic, JE Bruce, and RD Smith. 1999. "High Throughput Proteome-Wide Precision Measurements of Protein Expression using Mass Spectrometry." Journal of the American Chemical Society 121(34):7949-7950. 

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