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Staff information


Aaron Wright

Biological Sciences Division
Biomedical Scientist
Pacific Northwest National Laboratory
PO Box 999
MSIN: J4-02
Richland, WA 99352


Dr. Aaron Wright is a Senior Scientist and the Associate Director of the Biological Sciences Division and Director of the Biological Systems Science Group at PNNL. He also holds a joint appointment as a research professor in the Gene and Linda Voiland School of Chemical Engineering and Bioengineering at WSU. Aaron earned his Ph.D. in Organic Chemistry from the University of Texas at Austin with Prof. Eric Anslyn, and then carried out a postdoctoral fellowship in Chemical Biology with Prof. Benjamin Cravatt at The Scripps Research Institute. At PNNL, Aaron's research has focused on new chemical biology developments that are enabling function-driven research to improve our understanding of: (i) the impact of chemical exposures on drug and xenobiotic metabolism at the host-gut microbiome interface; (ii) the role that chemical exposures in early human development have on later life disease outcome that arise as a result of perturbations to the gut microbiome; (iii) microbial spatial and metabolic interactions in complex microbiomes; and (iv) mapping protein and receptor interactions of drugs, vitamins, opioids, and other small molecules of interest.

For a more comprehensive profile of Aaron’s research, see:

Research Interests

  • Chemical Biology
  • Activity-Based Protein Profiling
  • Microbiomes
  • Drug and Xenobiotic Metabolism
  • Proteomics

Education and Credentials

  • Postdoctoral Research Fellow, The Scripps Research Institute
  • Ph.D., Organic Chemistry, The University of Texas
  • B.S., Chemistry, George Fox University

Affiliations and Professional Service

  • American Chemical Society
  • Society of Toxicology

Awards and Recognitions

  • ACS Young Investigator Award
  • California Breast Cancer Research Program Postdoctoral Fellow
  • Dorothy Banks Graduate Research Fellow
  • University of Texas Career Development Grant
  • Dick van Santen Award, American Chemical Society
  • Edwards-Holman Science Award
  • National Science Foundation - Research Experience for Undergraduates Fellow - The University of Washington

PNNL Publications


  • Putnam E., J. Abellon-Ruiz, B.J. Killinger, J.J. Rosnow, A. Wexler, E. Folta-Stogniew, and A.T. Wright, et al. 2022. "Gut commensal Bacteroidetes encode a novel class of vitamin B12-binding proteins." mBio 13, no. 2:Art. No. e02845-21. PNNL-SA-167245. doi:10.1128/mbio.02845-21


  • Jarsberg L.G., K. Kedia, T.G. Wendler, A.T. Wright, P.D. Piehowski, M.A. Gritsenko, and T. Shi, et al. 2021. "Nutritional markers and proteome in patients undergoing treatment for pulmonary tuberculosis differ by geographic region." PLOS ONE 16, no. 5:Article No. e0250586. PNNL-SA-156554. doi:10.1371/journal.pone.0250586
  • Schultz K.J., S.M. Colby, V.S. Lin, A.T. Wright, and R.S. Renslow. 2021. "Ligand- and Structure-Based Analysis of Deep Learning-Generated Potential alpha2a Adrenoceptor Agonists." Journal of Chemical Information and Modeling 61, no. 1:481-492. PNNL-SA-155859. doi:10.1021/acs.jcim.0c01019
  • Stoddard E.G., S. Nag, J. Martin, K.J. Tyrrell, T.M. Gibbins, K.A. Anderson, and A.K. Shukla, et al. 2021. "Exposure to an environmental mixture of polycyclic aromatic hydrocarbons induces hepatic cytochrome P450 enzymes in mice." Chemical Research in Toxicology 34, no. 9:2145 - 2156. PNNL-SA-163871. doi:10.1021/acs.chemrestox.1c00235
  • Swift C., K.B. Louie, B.P. Bowen, H.M. Olson, S.O. Purvine, A. Salamov, and S.J. Mondo, et al. 2021. "Anaerobic gut fungi are an untapped reservoir of natural products." Proceedings of the National Academy of Sciences of the United States of America 118, no. 18:e2019855118. PNNL-SA-160632. doi:10.1073/pnas.2019855118
  • Wright A.T. 2021. "A transcriptional relationship with a natural product disrupts mitochondrial biogenesis." Cell Chemical Biology 28, no. 10:1392 - 1393. PNNL-SA-166818. doi:10.1016/j.chembiol.2021.10.002
  • Zegeye E., N.C. Sadler, G.X. Lomas, I.K. Attah, J.K. Jansson, K.S. Hofmockel, and C.R. Anderton, et al. 2021. "Activity-based protein profiling of chitin catabolism." Chembiochem 22, no. 4:717-723. PNNL-SA-156004. doi:10.1002/cbic.202000616


  • Killinger B.J., V.A. Petyuk, and A.T. Wright. 2020. "Detecting differential protein abundance by combining peptide level P-values." Molecular Omics 16, no. 6:554-562. PNNL-SA-144005. doi:10.1039/d0mo00045k
  • Steiger A.K., S.J. Fansler, C. Whidbey, C.J. Miller, and A.T. Wright. 2020. "Probe-Enabled Approaches for Function-Dependent Cell Sorting and Characterization of Microbiome Subpopulations." In Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems: Methods in Enzymology, edited by D.M. Chenoweth. 89-107. San Diego, California:Academic Press. PNNL-SA-150390. doi:10.1016/bs.mie.2020.03.014
  • Sveistyte A., T.M. Gibbins, K.J. Tyrrell, C.J. Miller, M.H. Foley, A.E. Plymale, and A.T. Wright, et al. 2020. "Simple analysis of primary and secondary bile salt hydrolysis in mouse and human gut microbiome samples using fluorogenic substrates." Chembiochem 21, no. 24:3539-3543. PNNL-SA-152472. doi:10.1002/cbic.202000370


  • Brandvold K.R., J.M. Weaver, C. Whidbey, and A.T. Wright. 2019. "A continuous fluorescence assay for simple quantification of bile salt hydrolase activity in the gut microbiome." Scientific Reports 9. PNNL-SA-133128. doi:10.1038/s41598-018-37656-7
  • Dickinson M., L.N. Anderson, B.M. Webb-Robertson, J.R. Hansen, R.D. Smith, A.T. Wright, and K. Hybiske. 2019. "Proximity-dependent proteomics of the Chlamydia trachomatis inclusion membrane reveals functional interactions with endoplasmic reticulum exit sites." PLoS Pathogens 15, no. 4:Article Number e1007698. PNNL-SA-132851. doi:10.1371/journal.ppat.1007698
  • Stoddard E.G., B.J. Killinger, S. Nag, J. Martin, R.A. Corley, J.N. Smith, and A.T. Wright. 2019. "Benzo[a]pyrene Induction of Glutathione S-transferases: An activity-based protein profiling investigation." Chemical Research in Toxicology 32, no. 6:1259-1267. PNNL-SA-137670. doi:10.1021/acs.chemrestox.9b00069
  • Whidbey C., N.C. Sadler, R.N. Nair, R.F. Volk, A.J. DeLeon, L.M. Bramer, and S.J. Fansler, et al. 2019. "A Probe-Enabled Approach for the Selective Isolation and Characterization of Functionally Active Subpopulations in the Gut Microbiome." Journal of the American Chemical Society 141, no. 1:42-47. PNNL-SA-127532. doi:10.1021/jacs.8b09668
  • Wright A.T. 2019. "Gut commensals make choline too." Nature Microbiology 4, no. 1:4-5. PNNL-SA-139558. doi:10.1038/s41564-018-0325-1
  • Zegeye E. 2019. "Selection, Succession, and Stabilization of Soil Microbial Consortia." mSystems 4, no. 4:Article Number e00055-19. PNNL-SA-146173. doi:10.1128/mSystems.00055-19
  • Zegeye E., C.J. Brislawn, Y. Farris, S.J. Fansler, K.S. Hofmockel, J.K. Jansson, and A.T. Wright, et al. 2019. "Selection, succession and stabilization of soil microbial consortia." mSystems 4, no. 4:e00055-19. PNNL-SA-138630. doi:10.1128/mSystems.00055-19
  • Zegeye E., C.J. Brislawn, Y. Farris, S.J. Fansler, K.S. Hofmockel, J.K. Jansson, and A.T. Wright, et al. 2019. "Selection, Succession, and Stabilization of Soil Microbial Consortia." mSystems 4, no. 4:Article Number e00055-19. PNNL-SA-146173. doi:10.1128/mSystems.00055-19


  • Kedia K., J.P. Wendler, E.M. Baker, K.E. Burnum-Johnson, L.G. Jarsberg, K.G. Stratton, and A.T. Wright, et al. 2018. "Application of multiplexed ion mobility spectrometry towards the identification of host protein signatures of treatment effect in pulmonary tuberculosis." Tuberculosis 112. PNNL-SA-137966. doi:10.1016/
  • Ortega C., A. Frando, B.M. Webb-Robertson, L.N. Anderson, N. Fleck, E.L. Flannery, and M. Fishbaugher, et al. 2018. "A global survey of ATPase activity in Plasmodium falciparum asexual blood stages and gametocytes." Molecular & Cellular Proteomics 17, no. 1:111-120. PNNL-SA-121236. doi:10.1074/mcp.RA117.000088
  • Rosnow J.J., S. Hwang, B.J. Killinger, Y. Kim, R.J. Moore, S.R. Lindemann, and J.A. Maupin-Furlow, et al. 2018. "A cobalamin activity-based probe enables microbial cell growth and finds new cobalamin-protein interactions across domains." Applied and Environmental Microbiology 84, no. 18:e00955-18. PNNL-SA-127734. doi:10.1128/AEM.00955-18
  • Sadler N.C., B.M. Webb-Robertson, T. Clauss, J.G. Pounds, R.A. Corley, and A.T. Wright. 2018. "High-fat diets alter the modulatory effects of xenobiotics on cytochrome P450 activities." Chemical Research in Toxicology 31, no. 5:308-318. PNNL-SA-126308. doi:10.1021/acs.chemrestox.8b00008
  • Stoddard E.G., R.F. Volk, J.P. Carson, C.M. Ljungberg, T.A. Murphree, J.N. Smith, and N.C. Sadler, et al. 2018. "Multifunctional Activity-Based Protein Profiling of the Developing Lung." Journal of Proteome Research 17, no. 8:2623-2634. PNNL-SA-132239. doi:10.1021/acs.jproteome.8b00086
  • Whidbey C., and A.T. Wright. 2018. "Activity-based protein profiling - enabling multimodal functional studies of microbial communities." In Activity-Based Protein Profiling: Current Topics in Microbiology and Immunology", edited by B.F. Cravatt, K.L. Hsu, and E. Weerapana. 1-21. Cham:Springer. PNNL-SA-136518. doi:10.1007/82_2018_128


  • Haitjema C., S.P. Gilmore, J.K. Henske, K.V. Solomon, R.D. Groot, A. Kuo, and S.J. Mondo, et al. 2017. "A Parts List for Fungal Cellulosomes Revealed by Comparative Genomics." Nature Microbiology 2. PNNL-SA-120227. doi:10.1038/nmicrobiol.2017.87
  • Nair R.N., J.J. Rosnow, T.A. Murphree, M.E. Bowden, S.R. Lindemann, and A.T. Wright. 2017. "De novo synthesis of alkyne substituted tryptophans as chemical probes for protein profiling studies." Organic Chemistry Frontiers 4, no. 4:495-499. PNNL-SA-122250. doi:10.1039/C6QO00819D
  • Romine M.F., D.A. Rodionov, Y. Maezato, L.N. Anderson, P. Nandhikonda, I.A. Rodionova, and A. Carre, et al. 2017. "Elucidation of new roles for vitamin B12 in regulation of folate, ubiquinone, and methionine metabolism." Proceedings of the National Academy of Sciences of the United States of America 114, no. 7:E1205-E1214. PNNL-SA-119181. doi:10.1073/pnas.1612360114
  • Rosnow J.J., L.N. Anderson, R.N. Nair, E.S. Baker, and A.T. Wright. 2017. "Profiling microbial lignocellulose degradation and utilization by emergent omics technologies." Critical Reviews in Biotechnology 37, no. 5:626-640. PNNL-SA-116817. doi:10.1080/07388551.2016.1209158
  • Smith J.N., K.J. Tyrrell, J.R. Hansen, D.G. Thomas, T.A. Murphree, A.K. Shukla, and T. Luders, et al. 2017. "Plasma Protein Turnover Rates in Rats Using Stable Isotope Labeling, Global Proteomics, and Activity-Based Protein Profiling." Analytical Chemistry 89, no. 24:13559-13566. PNNL-SA-129561. doi:10.1021/acs.analchem.7b03984
  • Stoddard E.G., B.J. Killinger, R.N. Nair, N.C. Sadler, R.F. Volk, S.O. Purvine, and A.K. Shukla, et al. 2017. "Activity-Based Probes for Isoenzyme- and Site-Specific Functional Characterization of Glutathione S- Transferases." Journal of the American Chemical Society 139, no. 45:16032-16035. PNNL-SA-127421. doi:10.1021/jacs.7b07378


  • Anderson L.N., P.K. Koech, A.E. Plymale, E.V. Landorf, A. Konopka, F. Collart, and M.S. Lipton, et al. 2016. "Live Cell Discovery of Microbial Vitamin Transport and Enzyme-Cofactor Interactions." ACS Chemical Biology 11, no. 2:345-354. PNNL-SA-108145. doi:10.1021/acschembio.5b00918
  • Bennett K., N.C. Sadler, A.T. Wright, C. Yeager, and M.R. Hyman. 2016. "Activity-based protein profiling of ammonia monooxygenase in Nitrosomonas europaea." Applied and Environmental Microbiology 82, no. 8:2270-2279. PNNL-SA-114336. doi:10.1128/AEM.03556-15
  • Biteen J.S., P.C. Blainey, Z.G. Cardon, M. Chun, G. Church, P.C. Dorrestein, and S.E. Fraser, et al. 2016. "Tools for the Microbiome: Nano and Beyond." ACS Nano 10, no. 1:6-37. PNNL-SA-115178. doi:10.1021/acsnano.5b07826
  • Ortega C., L.N. Anderson, A. Frando, N.C. Sadler, R.W. Brown, R.D. Smith, and A.T. Wright, et al. 2016. "Systematic Survey of Serine Hydrolase Activity in Mycobacterium tuberculosis Defines Changes Associated with Persistence." Cell Chemical Biology 23, no. 2:290-298. PNNL-SA-112430. doi:10.1016/j.chembiol.2016.01.003
  • Perez D.M., M.E. Richards, R. Parker, M.E. Berres, A.T. Wright, M. Sifri, and N.C. Sadler, et al. 2016. "Role of cytochrome P450 hydroxylase in the decreased accumulation of vitamin E in muscle from turkeys compared to that from chickens." Journal of Agricultural and Food Chemistry 64, no. 3:671-680. PNNL-SA-108079. doi:10.1021/acs.jafc.5b05433
  • Sadler N.C., H.C. Bernstein, M.R. Melnicki, M.A. Charania, E.A. Hill, L.N. Anderson, and M.E. Monroe, et al. 2016. "Dinitrogenase Driven Photobiological Hydrogen Production Combats Oxidative Stress in Cyanothece sp. ATCC 51142." Applied Environmental Microbiology 82, no. 24:7227-7235. PNNL-SA-113160. doi:10.1128/AEM.02098-16
  • Sadler N.C., P. Nandhikonda, B.M. Webb-Robertson, C. Ansong, L.N. Anderson, J.N. Smith, and R.A. Corley, et al. 2016. "Hepatic cytochrome P450 activity, abundance, and expression throughout human development." Drug Metabolism and Disposition 44, no. 7:984-91. PNNL-SA-112422. doi:10.1124/dmd.115.068593
  • Solomon K.V., C. Haitjema, J.K. Henske, S.P. Gilmore, D. Borges-Rivera, A. Lipzen, and H.M. Brewer, et al. 2016. "Early-branching Gut Fungi Possess A Large, And Comprehensive Array Of Biomass-Degrading Enzymes." Science 351, no. 6278:1192-1195. PNNL-SA-115073. doi:10.1126/science.aad1431
  • Wright A.T. 2016. "HOST-PATHOGEN INTERACTIONS: A cholera surveillance system." Nature Chemical Biology 12, no. 4:203-204. PNNL-SA-115724. doi:10.1038/nchembio.2039


  • Bernstein H.C., M.A. Charania, R.S. McClure, N.C. Sadler, M.R. Melnicki, E.A. Hill, and L.M. Markillie, et al. 2015. "Multi-omic Dynamics Associate Oxygenic Photosynthesis with Nitrogenase-mediated H2 production in Cyanothece sp. ATCC 51142." Scientific Reports 5. PNNL-SA-111925. doi:10.1038/srep16004
  • Liu Y., J.K. Fredrickson, N.C. Sadler, P. Nandhikonda, R.D. Smith, and A.T. Wright. 2015. "Advancing Understanding of Microbial Bioenergy Conversion Processes by Activity-Based Protein Profiling." Biotechnology for Biofuels 8. PNNL-SA-107650. doi:10.1186/s13068-015-0343-7
  • Sadler N.C., and A.T. Wright. 2015. "Activity-Based Protein Profiling of Microbes." Current Opinion in Chemical Biology 24. PNNL-SA-105009. doi:10.1016/j.cbpa.2014.10.022
  • Wright A.T., T. Magnaldo, R.L. Sontag, L.N. Anderson, N.C. Sadler, P.D. Piehowski, and Y. Gache, et al. 2015. "Deficient Expression of Aldehyde Dehydrogenase 1A1 Is Consistent with Increased Sensitivity of Gorlin Syndrome Patients to Radiation Carcinogenesis." Molecular Carcinogenesis 54, no. 6:473-484. PNNL-SA-95210. doi:10.1002/mc.22115


  • Ansong C., N.C. Sadler, E.A. Hill, M.P. Lewis, E.M. Zink, R.D. Smith, and A.S. Beliaev, et al. 2014. "Characterization of protein redox dynamics induced during light-to-dark transitions and nutrient limitation in cyanobacteria." Frontiers in Microbiology 5. PNNL-SA-101966. doi:10.3389/fmicb.2014.00325
  • Ismail H.M., P.M. O'Neill, D. Hong, R. Finn, C. Henderson, A.T. Wright, and B. Cravatt, et al. 2014. "Pyrethroid Activity-Based Probes for Profiling Cytochrome P450 Activities Associated with Insecticide Interactions." Proceedings of the National Academy of Sciences of the United States of America 110, no. 49:19766-19771. PNNL-SA-98703. doi:10.1073/pnas.1320185110
  • Liu Y., R. Zhang, Z. Lian, S. Wang, and A.T. Wright. 2014. "Yeast cell surface display for lipase whole cell catalyst and its applications." Journal of Molecular Catalysis B: Enzymatic 106. PNNL-SA-102321. doi:10.1016/j.molcatb.2014.04.011
  • Ortega C., R. Liao, L.N. Anderson, T. Rustad, A.R. Ollodart, A.T. Wright, and D.R. Sherman, et al. 2014. "Mycobacterium tuberculosis Ser/Thr protein kinase B mediates an oxygen-dependent replication switch." PLoS Biology 12, no. 1:Article No. e1001746. PNNL-SA-94282. doi:10.1371/journal.pbio.1001746
  • Pena-Castillo L., R. Mercer, A. Gurinovich, S.J. Callister, A.T. Wright, A. Westbye, and J.T. Beatty, et al. 2014. "Gene co-expression network analysis in Rhodobacter capsulatus and application to comparative expression analysis of Rhodobacter sphaeroides." BMC Genomics 15. PNNL-SA-105663. doi:10.1186/1471-2164-15-730
  • Sadler N.C., M.R. Melnicki, M.H. Serres, E.D. Merkley, W.B. Chrisler, E.A. Hill, and M.F. Romine, et al. 2014. "Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium." ACS Chemical Biology 9, no. 1:291-300. PNNL-SA-93812. doi:10.1021/cb400769v
  • Wiedner S.D., L.N. Anderson, N.C. Sadler, W.B. Chrisler, V.K. Kodali, R.D. Smith, and A.T. Wright. 2014. "Organelle-Specific Activity-Based Protein Profiling in Living Cells." Angewandte Chemie International Edition 53, no. 11:2919-2922. PNNL-SA-98713. doi:10.1002/anie.201309135


  • Anderson L.N., D.E. Culley, B.A. Hofstad, L.M. Chauvigne-Hines, E.M. Zink, S.O. Purvine, and R.D. Smith, et al. 2013. "Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30." Molecular Biosystems 9, no. 12:2992-3000. PNNL-SA-97265. doi:10.1039/c3mb70333a
  • Ansong C., C. Ortega, S.H. Payne, D.H. Haft, L.M. Chauvigne-Hines, M.P. Lewis, and A.R. Ollodart, et al. 2013. "Identification of widespread adenosine nucleotide binding in Mycobacterium tuberculosis." Chemistry & Biology 20, no. 1:123-133. PNNL-SA-88963. doi:10.1016/j.chembiol.2012.11.008
  • Crowell S.R., A. Sharma, S. Amin, J.J. Soelberg, N.C. Sadler, A.T. Wright, and W.M. Baird, et al. 2013. "Impact of Pregnancy on the Pharmacokinetics of Dibenzo[def,p]chrysene in Mice." Toxicological Sciences 135, no. 1:48-62. PNWD-SA-10207. doi:10.1093/toxsci/kft124
  • Wiedner S.D., C. Ansong, B.M. Webb-Robertson, L.M. Pederson, S. Fortuin, B.A. Hofstad, and A.K. Shukla, et al. 2013. "Disparate Proteome Responses of Pathogenic and Non-pathogenic Aspergilli to Human Serum Measured by Activity-Based Protein Profiling (ABPP)." Molecular & Cellular Proteomics. MCP 12, no. 7:1791-1805. PNNL-SA-91969. doi:10.1074/mcp.M112.026534


  • Chauvigne-Hines L.M., L.N. Anderson, H.M. Weaver, J.N. Brown, P.K. Koech, C.D. Nicora, and B.A. Hofstad, et al. 2012. "Suite of Activity-Based Probes for Cellulose-Degrading Enzymes." Journal of the American Chemical Society 134, no. 50:20521-20532. PNNL-SA-91066. doi:10.1021/ja309790w
  • Sadler N.C., T.E. Angel, M.P. Lewis, L.M. Pederson, L.M. Chauvigne-Hines, S.D. Wiedner, and E.M. Zink, et al. 2012. "Activity-Based Protein Profiling Reveals Mitochondrial Oxidative Enzyme Impairment and Restoration in Diet-Induced Obese Mice." PLoS One 7, no. 10:Article No. e47996. PNNL-SA-88011. doi:10.1371/journal.pone.0047996
  • Wiedner S.D., K.E. Burnum, L.M. Pederson, L.N. Anderson, S. Fortuin, L.M. Chauvigne-Hines, and A.K. Shukla, et al. 2012. "Multiplexed Activity-based Protein Profiling of the Human Pathogen Aspergillus fumigatus Reveals Large Functional Changes upon Exposure to Human Serum." Journal of Biological Chemistry 287, no. 40:33447-33459. PNNL-SA-87258. doi:10.1074/jbc.M112.394106

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