Dr. Zhou is a chemist with the Biomolecular Pathways team in PNNL’s Environmental Molecular Sciences Division and the Environmental Molecular Sciences Laboratory (EMSL) user program. His research focuses on development of novel mass spectrometry techniques to understand proteins/genes with unknown function. Dr. Zhou has developed native mass spectrometry methods to study higher-order structures of unknown enzymes from microbes and plants. He also developed top-down mass spectrometry methods to characterize unknown post-translational modifications, especially histones that are involved in epigenetic control of gene expression. 


Research Interest

  • Proteins/genes within unknown function 

  • Protein post-translational modifications 

  • Native mass spectrometry 

  • Top-down proteomics 

  • Structural biology 


  • PhD in Analytical Chemistry, Ohio State University, 2013  

  • Doctoral Student in Analytical Chemistry, University of Arizona, 2008 – 2012  

  • BS in Chemistry, Wuhan University, 2008 

Affiliations and Professional Service

  • American Society for Mass Spectrometry 

Awards and Recognitions

  • College of Science Galileo Circle Scholarship, University of Arizona (2012) 



  • Srzentic K., L. Fornelli, Y.O. Tsybin, J. Loo, H. Seckler, J.N. Agar, and L.C. Anderson, et al. 2020. "Interlaboratory Study for Characterizing Monoclonal Antibodies by Top-Down and Middle-Down Mass Spectrometry." Journal of the American Society for Mass Spectrometry 31, no. 9:1783-1802. PNNL-SA-147397. doi:10.1021/jasms.0c00036 

  • Zhou L., M. Zhou, M.A. Gritsenko, and G. Stacey. 2020. "Selective Enrichment Coupled with Proteomics to Identify S-Acylated Plasma Membrane Proteins in Arabidopsis." Current Protocols in Plant Biology 5, no. 4:Article No. e20119. PNNL-SA-155437. doi:10.1002/cppb.20119 

  • Zhou M., N.O. Uwugiaren, S.M. Williams, R.J. Moore, R. Zhao, D. Goodlett, and I. Dapic, et al. 2020. "Sensitive Top-Down Proteomics Analysis of Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform." Analytical Chemistry 92, no. 10:7087-7095. PNNL-SA-149823. 

  • Zhou M., W. Liu, and J.B. Shaw. 2020. "Charge Movement and Structural Changes in the Gas-Phase Unfolding of Multimeric Protein Complexes Captured by Native Top-Down Mass Spectrometry." Analytical Chemistry 92, no. 2:1788-1795. PNNL-SA-145158. doi:10.1021/acs.analchem.9b03469 


  • Garcellano R.C., S. Moinuddin, R.P. Young, M.E. Bowden, M. Zhou, R.S. Renslow, and Y. Yesiltepe, et al. 2019. "Isolation of Tryptanthrin and Reassessment of Evidence for its Isobaric Isostere Wrightiadione in Plants of the Wrightia Genus." Journal of Natural Products 82, no. 3:440-448. PNNL-SA-135279. doi:10.1021/acs.jnatprod.8b00567 

  • Harvey, S. R., J. T. Seffernick, R. S. Quintyn, Y. Song, Y. Ju, J. Yan, A. N. Sahasrabuddhe, A. Norris, M. Zhou, E. J. Behrman, S. Lindert, and V. H. Wysocki. 2019. “Relative Interfacial Cleavage Energetics of Protein Complexes Revealed by Surface Collisions.” Proceedings of the National Academy of Sciences of the United States of America 116 (17): 8143-8148.   

  • Nagarajan A., M. Zhou, A.Y. Nguyen, M.L. Liberton, K. Kedia, T. Shi, and P.D. Piehowski, et al. 2019. "Proteomic insights into phycobilisome degradation, a selective and tightly controlled process in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Biomolecules 9, no. 8:Article Number 374. PNNL-SA-146394. doi:10.3390/biom9080374 

  • Zhou, M., N. Malhan, A. H. Ahkami, K. Engbrecht, G. Myers, J. Dahlberg, J. Hollingsworth, J. A. Sievert, R. Hutmacher, M. Madera, P. G. Lemaux, K. K. Hixson, C. Jansson, and L. Paša-Tolić. 2019. “Top-Down Mass Spectrometry of Histone Modifications in Sorghum Reveals Potential Epigenetic Markers for Drought Acclimation.” Methods.   


  • Gargano A., J.B. Shaw, M. Zhou, C.S. Wilkins, T.L. Fillmore, R.J. Moore, and G.W. Somsen, et al. 2018. "Increasing the Separation Capacity of Intact Histone Proteoforms Chromatography Coupling Online Weak Cation Exchange-HILIC to Reversed Phase LC UVPD-HRMS." Journal of Proteome Research 17, no. 11:3791-3800. PNNL-SA-138847. doi:10.1021/acs.jproteome.8b00458 

  • Park, J., P. D. Piehowski, C. Wilkins, M. Zhou, J. Mendoza, G. M. Fujimoto, B. C. Gibbons, J. B. Shaw, Y. Shen, A. K. Shukla, R. J. Moore, T. Liu, V. A. Petyuk, N. Tolić, L. Paša-Tolić, R. D. Smith, S. H. Payne, and S. Kim. 2018. “Author Correction: Informed-Proteomics: Open-Source Software Package for Top-Down Proteomics.” Nature Methods 15 (7): 554.   

  • Zhou M., J. Yan, C.A. Romano, B.M. Tebo, V.H. Wysocki, and L. Pasa Tolic. 2018. "Surface Induced Dissociation Coupled with High Resolution Mass Spectrometry Unveils Heterogeneity of a 211 kDa Multicopper Oxidase Protein Complex." Journal of the American Society for Mass Spectrometry 29, no. 4:723-733. PNNL-SA-124854. doi:10.1007/s13361-017-1882-x 


  • Park J., P.D. Piehowski, C.S. Wilkins, M. Zhou, J.A. Mendoza, G.M. Fujimoto, and B.C. Gibbons, et al. 2017. "Informed-Proteomics: Open Source Software Package for Top-down Proteomics." Nature Methods 14, no. 9:909-914. PNNL-SA-120171. doi:10.1038/nmeth.4388 

  • Romano C.A., M. Zhou, Y. Song, V.H. Wysocki, A. Dohnalkova, L. Kovarik, and L. Pasa Tolic, et al. 2017. "Biogenic manganese oxide nanoparticle formation by a multimeric multicopper oxidase Mnx." Nature Communications 8. PNNL-SA-124759. doi:10.1038/s41467-017-00896-8 

  • Yan J., M. Zhou, J. Gilbert, J.J. Wolff, A. Somogyi, R.E. Pedder, and R.S. Quintyn, et al. 2017. "Surface-induced dissociation of protein complexes in a hybrid Fourier transform ion cyclotron resonance mass spectrometer." Analytical Chemistry 89, no. 1:895-901. PNNL-SA-121761. doi:10.1021/acs.analchem.6b03986 

  • Zhou M., L. Pasa Tolic, and D.L. Stenoien. 2017. "Profiling of Histone Post-translational Modifications in Mouse Brain with High Resolution Top Down Mass Spectrometry." Journal of Proteome Research 16, no. 2:599-608. PNNL-SA-119705. doi:10.1021/acs.jproteome.6b00694 

  • Zhou, M., S. Wu, D. L. Stenoien, Z. Zhang, L. Connolly, M. Freitag, and L. Paša-Tolić. 2017. Profiling Changes in Histone Post-Translational Modifications by Top-Down Mass Spectrometry. In Methods in Molecular Biology. 


  • Quintyn, R. S., M. Zhou, J. Yan, and V. H. Wysocki. 2015. “Surface-Induced Dissociation Mass Spectra as a Tool for Distinguishing Different Structural Forms of Gas-Phase Multimeric Protein Complexes.” Analytical Chemistry 87 (23): 11879-11886.   

  • Zhou, M., A. C. Gucinski, and M. T. Boyne, II. 2015. “Performance Metrics for Evaluating System Suitability in Liquid Chromatography—Mass Spectrometry Peptide Mass Mapping of Protein Therapeutics and Monoclonal Antibodies.” mAbs 7 (6): 1104-1117.


  • Ihms, E. C., M. Zhou, Y. Zhang, I. R. Kleckner, C. A. McElroy, V. H. Wysocki, P. Gollnick, and M. P. Foster. 2014. “Gene Regulation by Substoichiometric Heterocomplex Formation of Undecameric TRAP and Trimeric Anti-TRAP.” Proceedings of the National Academy of Sciences of the United States of America 111 (9): 3442-3447.   

  • Ma, X., M. Zhou, and V. H. Wysocki. 2014. “Surface Induced Dissociation Yields Quaternary Substructure of Refractory Noncovalent Phosphorylase B and Glutamate Dehydrogenase Complexes.” Journal of the American Society for Mass Spectrometry 25 (3): 368-379.   

  • Zhou, M., and V. H. Wysocki. 2014. “Surface Induced Dissociation: Dissecting Noncovalent Protein Complexes in the Gas Phase.” Accounts of Chemical Research 47 (4): 1010-1018.


  • Ma, X., S. Shah, M. Zhou, C. K. Park, V. H. Wysocki, and N. C. Horton. 2013. “Structural Analysis of Activated SgrAI-DNA Oligomers Using Ion Mobility Mass Spectrometry.” Biochemistry 52 (25): 4373-4381.   

  • Mashalidis, E. H., D. B. Briggs, M. Zhou, A. M. Vergara, J. J. Chhun, R. K. Ellsworth, R. M. Giron, J. Rood, G. A. Bray, S. R. Smith, V. H. Wysocki, and T. S. Tsao. 2013. “High-Resolution Identification of Human Adiponectin Oligomers and Regulation by Pioglitazone in Type 2 Diabetic Patients.” Analytical Biochemistry 437 (2): 150-160.   

  • Quintyn, R. S., M. Zhou, S. Dagan, J. Finke, and V. H. Wysocki. 2013. “Ligand Binding and Unfolding of Tryptophan Synthase Revealed by Ion Mobility-Tandem Mass Spectrometry Employing Collision and Surface Induced Dissociation.” International Journal for Ion Mobility Spectrometry 16 (2): 133-143.   

  • Zhou, M., S. Dagan, and V. H. Wysocki. 2013. “Impact of Charge State on Gas-Phase Behaviors of Noncovalent Protein Complexes in Collision Induced Dissociation and Surface Induced Dissociation.” Analyst 138 (5): 1353-1362.  

  • Zhou, M., C. M. Jones, and V. H. Wysocki. 2013. “Dissecting the Large Noncovalent Protein Complex GroEL with Surface-Induced Dissociation and Ion Mobility-Mass Spectrometry.” Analytical Chemistry 85 (17): 8262-8267.  


  • Mealman, T. D., M. Zhou, T. Affandi, K. N. Chacón, M. E. Aranguren, N. J. Blackburn, V. H. Wysocki, and M. M. McEvoy. 2012. “N-Terminal Region of CusB Is Sufficient for Metal Binding and Metal Transfer with the Metallochaperone CusF.” Biochemistry 51 (34): 6767-6775.   

  • Zhou, M., S. Dagan, and V. H. Wysocki. 2012. “Protein Subunits Released by Surface Collisions of Noncovalent Complexes: Nativelike Compact Structures Revealed by Ion Mobility Mass Spectrometry.” Angewandte Chemie - International Edition 51 (18): 4336-4339.   

  • Zhou, M., C. Huang, and V. H. Wysocki. 2012. “Surface-Induced Dissociation of Ion Mobility-Separated Noncovalent Complexes in a Quadrupole/Time-of-Flight Mass Spectrometer.” Analytical Chemistry 84 (14): 6016-6023.