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Biological Sciences

Facilities/Capabilities

facilities Uses

Radiation Facilities

Radiation activities available at PNNL include

  • 250 kVp x-ray cabinet
  • x-ray facility with over 70 NIST and ISO beams ranging from 8-250 keV
  • Gamma facility
  • Neutron low-scatter facility
  • Low dose-rate facilities
  • Electron microbeam for spatially resolved exposures
  • Very-low dose rate studies, a unique capability at PNNL

Proteomics

PNNL's cutting-edge proteomics tools and methods to study cell and tissue responses to LDR include

  • Global analysis of complex protein mixtures
  • Targeted analysis of sub-cellular compartments
  • Quantitation with isotopic labeling and label-free methods
  • PTM identification and quantitation
  • Intact protein analysis methods

Metabolomics

Tools and methods for metabolomics include

  • Targeted metabolic analysis using triple-quadrupole and quadrupole-ion-trap mass spectrometry (MS)
  • Global metabolomics and lipidomics using gas chromatography and LTQ-Orbitrap MS
  • Metabolite identification and quantification

Genomics

Capabilities include

  • De novo sequencing
  • Whole genome sequencing
  • Targeted resequencing
de novo

Epigenomics

  • Chromatin immunoprecipitation sequencing (ChIP seq)
  • Methylation analysis

Transcriptomics

  • Gene expression profiling
  • Whole transcriptomic analysis
  • Small RNA analysis

Imaging and Cell Isolation

Capabilities include

  • Real-time confocal microscopy
  • Live cell imaging
  • Laser capture microdissection
  • Multi-photon imaging
  • Mass-spectrometry-based imaging
  • Flow cytometry
imaging

Protein Microarrays

  • Excellent sensitivity as low as 1 pg/ml
  • High throughput
  • Simultaneous measurement of up to 50 proteins
  • In-house software (ProMat) for rapid and statistical analysis of protein microarray data
Cova

PNNL's Low Dose Radiation Research Program

Biology

Fundamental & Computational Sciences

Resources

Contacts

DOE Program Manager
Noelle Metting

BER Sector Manager
Harvey Bolton

Principal Investigator
Bill Morgan

Science Advisor
Tony Brooks

Communications